User: xioli2013

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xioli20130
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Posts by xioli2013

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Comment: C: How to map taxonomy to OTUs
... Thanks for the reply. I am using trnL as described in the question. I have solved the issue now. ...
written 9 months ago by xioli20130
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Answer: A: How to map taxonomy to OTUs
... I have solved the issue by creating right formatted id_to_taxonomy table and fed it to assign_taxonomy.py in QIIMME, which worked. ...
written 9 months ago by xioli20130
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How to map taxonomy to OTUs
... Hi, I have encountered an issue that I was trying to map taxonomy to an otu table. The barcode is trnL. I have managed to get all viridiplantae trnL sequences from NCBI as a database for local blast, and the associated lineage to each sequence entry. Also I have the representative sequences by vsea ...
otu trnl taxonomy table written 9 months ago by xioli20130
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formatting lineage to taxonomy file
... Hi, I am trying to make a taxonomy file like the one provided in qiime for all trnL gene. What I have done: 1. all trnL sequences 2. all trnL gene accession number 3. all trnL gene tax id I was able to get the lineage tag from the xml efetch function generated in Python: ``'1435590': 'cellular or ...
metabarcode taxonomy file formatting trnl written 10 months ago by xioli20130
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Comment: C: a confusion about pandaseq assembly
... Hi Kevin, thanks for the reply. One more question: Should the output of pandaseq assembly look like [forward primer][sequence][reverse primer] without trimming the primers? ...
written 10 months ago by xioli20130
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Comment: C: a confusion about pandaseq assembly
... Hi Kevin, here are the fastqc report, the quality of 3' is not good as it normally is for NGS [Foward QC][1] [Reverse QC][2] [1]: https://drive.google.com/open?id=1wee8D9nv_uiX8piqvBI0iLIBtHSIg9_a [2]: https://drive.google.com/open?id=19_srd3Z7gi5Z6mKiWGScXFTbz9n4IMXj What do you think woul ...
written 10 months ago by xioli20130
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Comment: C: a confusion about pandaseq assembly
... I played around the tools such as bbmerge and pandaseq with trimming and no trimming with trimmomatic 0.36 the # of raw reads in R1 is 194543 No trimming: ``$BBMerge in1=V1_R1.fastq in2=V1_R2.fastq out=bbmap_notrim_merged.fq outu=bbmap_notrim_unmerged.fq \`` ``adapters=NexteraPE-PE.fa ihist=ihist_ ...
written 10 months ago by xioli20130
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Comment: C: a confusion about pandaseq assembly
... Hi Kevin, I just added the links to the reads. Hope you can take a look at them. ...
written 10 months ago by xioli20130
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a confusion about pandaseq assembly
... Hi, I have a Miseq data with about 300 bp long, paired end reads, barcode is trnL(c)/UAA(h) The first step I am attempting is use pandaseq to assemble the pe reads this is the command line: ``pandaseq -f lane1-s001-indexN716-B-S502-B-ACTCGCTA-CTCTCTAT-V-1_S1 _L001_R1_001.fastq -r lane1-s001-index ...
pandaseq miseq written 10 months ago by xioli20130 • updated 10 months ago by Kevin Blighe30k
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Comment: C: Error when running Mothur MiSeqSOP: "Mock is not a valid group, and will be disr
... Have you solved the problem yet? I have encountered the exact same issue. I found out that the problem should be from the output of make.file command where it does not add the names of samples to the file. ...
written 11 months ago by xioli20130

Latest awards to xioli2013

Scholar 9 months ago, created an answer that has been accepted. For A: How to map taxonomy to OTUs

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