User: eyonesi

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Posts by eyonesi

<prev • 12 results • page 1 of 2 • next >
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Comment: C: Bimodal GC content
... Hello, dear thackl I was running a denovo rnaseq expriment on a plant.similarity, my fastq GC content result is bimodal. Is it possible for you to more explain about "the second peak corresponded to specific repeat class"? I think it is depended to existance of chloroplast genome, what is your idea? ...
written 24 days ago by eyonesi0
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Comment: C: How to interpret and trim this plots?
... thanks for your answer. Can I ignore all output even if it shows two peaks in gc content? with regards ...
written 25 days ago by eyonesi0
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How to interpret and trim this plots?
... hello everyone I am running denovo rnaseq experiment at quality control step. I can’t interpret and trim two plot duplication level and GC content at output of fastqc . I have read some articles that is not recommended to remove duplicates for differential expression analysis. I don’t know that ho ...
rna-seq written 25 days ago by eyonesi0 • updated 25 days ago by Michael Dondrup43k
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minimum hardware requirement for denovo RNAseq analysis
... Hi all Dear what are minimum hardware requirements (RAM, Hard Disk, CPU, Graphic Card) for denovo RNAseq analysis, when it is avaiable 32 Gb raw data, Trinity pipeline? ...
rna-seq written 5 months ago by eyonesi0 • updated 5 months ago by genomax34k
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Comment: C: to pool or not to pool
... dear WouterDeCoster thank you ...
written 6 months ago by eyonesi0
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Comment: A: to pool or not to pool
... exactly I asked about pooling extracted RNA from replicates of each treatments to create libraries. So which one is better ' pooling RNA or not pooling? and also if we pool the RNA, isn't it complicate the analysis and assembly in a de novo analysis? ...
written 7 months ago by eyonesi0
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Comment: A: to pool or not to pool
... thank you st.ph.n its OK I'm going to do DGE. but which method is preffered or is better? ...
written 7 months ago by eyonesi0
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to pool or not to pool
... Hi all in experimental design for de novo transcription analysis (de novo RNA seq analysis) which method is preferred' to pool or not to pool of replicates? thank you ...
rna-seq written 7 months ago by eyonesi0
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requirements for denovo rnaseq analysis
... Hi All Dear For analysis the rnaseq data without refrence genome (denovo) from a plant with genome size as equal as rice what hardware is need? In the other words, what is requirements of a pc for denovo rnaseq analysis? ...
rna-seq written 9 months ago by eyonesi0
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Comment: C: Is it possible to compare two samples that their RNA was extracted by two differ
... Thanks for your guidance. ...
written 10 months ago by eyonesi0

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