User: eyonesi
eyonesi • 0
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Posts by eyonesi
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Comment:
C: Bimodal GC content
... Hello, dear thackl
I was running a denovo rnaseq expriment on a plant.similarity, my fastq GC content result is bimodal. Is it possible for you to more explain about "the second peak corresponded to specific repeat class"? I think it is depended to existance of chloroplast genome, what is your idea? ...
written 24 days ago by
eyonesi • 0
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... thanks for your answer. Can I ignore all output even if it shows two peaks in gc content?
with regards
...
written 25 days ago by
eyonesi • 0
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... hello everyone
I am running denovo rnaseq experiment at quality control step. I can’t interpret and trim two plot duplication level and GC content at output of fastqc . I have read some articles that is not recommended to remove duplicates for differential expression analysis. I don’t know that ho ...
written 25 days ago by
eyonesi • 0
• updated
25 days ago by
Michael Dondrup ♦ 43k
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... Hi all Dear
what are minimum hardware requirements (RAM, Hard Disk, CPU, Graphic Card) for denovo RNAseq analysis, when it is avaiable 32 Gb raw data, Trinity pipeline? ...
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Comment:
C: to pool or not to pool
... dear WouterDeCoster
thank you
...
written 6 months ago by
eyonesi • 0
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Comment:
A: to pool or not to pool
... exactly I asked about pooling extracted RNA from replicates of each treatments to create libraries. So which one is better ' pooling RNA or not pooling? and also if we pool the RNA, isn't it complicate the analysis and assembly in a de novo analysis? ...
written 7 months ago by
eyonesi • 0
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Comment:
A: to pool or not to pool
... thank you st.ph.n
its OK I'm going to do DGE. but which method is preffered or is better? ...
written 7 months ago by
eyonesi • 0
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... Hi all
in experimental design for de novo transcription analysis (de novo RNA seq analysis) which method is preferred' to pool or not to pool of replicates?
thank you ...
written 7 months ago by
eyonesi • 0
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... Hi All Dear
For analysis the rnaseq data without refrence genome (denovo) from a plant with genome size as equal as rice what hardware is need? In the other words, what is requirements of a pc for denovo rnaseq analysis?
...
written 9 months ago by
eyonesi • 0
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... Thanks for your guidance. ...
written 10 months ago by
eyonesi • 0
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