User: Zhilong Jia
Zhilong Jia • 1.5k
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a bioinformatician
Posts by Zhilong Jia
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... Are there tools to infer population structure and evolution from structural variation genotype data?
Generally, [fastStructure][1] or other tools are designed for SNPs. Are inferring population structure and evolution from structural variation genotype data largely different from that from SNP gen ...
written 8 months ago by
Zhilong Jia • 1.5k
• updated
6 months ago by
Biostar ♦♦ 20
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Comment:
C: Downloading Data From Mg-Rast
... http://api.metagenomics.anl.gov/metadata/export/mgp128
ref: http://api-ui.mg-rast.org/api.html#sample ...
written 21 months ago by
Zhilong Jia • 1.5k
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Comment:
C: Downloading Data From Mg-Rast
... File 050.2 - This is the unfiltered metagenome that was originally uploaded to MG-RAST
File 100.1 - preprocess.passed.fna
File 100.2 - preprocess.removed (low quality)
File 350.2 & 350.3 - These are the protein coding genes (amino acids and nucleotides)
File 440.1 - These are ...
written 21 months ago by
Zhilong Jia • 1.5k
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... in line 4, it should be: ` foo <- data.frame(SampleType = paste0(rep(c("A","B"), each = 3)), Reps = rep(1:3, 2),` `Names = paste0(rep(c("A","B"), each=3), rep(1:3, 2) ) )`
`set.seed()` before generating the data will be helpful.
...
written 22 months ago by
Zhilong Jia • 1.5k
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2.2k
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Answer:
A: RNA-seq cluster analysis in R
... 0. [clusterProfiler][1]. [Visualization of profile comparison][2] section from Bioconductor package `clusterProfiler`. A quick solution for your question.
see ![figure][3].
1. [co-expressed gene set enrichment analysis, cogena][4]. `cogena` started with gene expression not clustered genes, While i ...
written 22 months ago by
Zhilong Jia • 1.5k
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... 0. [the toppcluster webserver][1], but not work recently.
1. [co-expressed gene set enrichment analysis, cogena][2]. But Zebrafish GO gene sets as a gmt file are needed.
2. clusterProfiler. `GO analyses (groupGO(), enrichGO() and gseGO()) support organisms that have an OrgDb object available.` so it ...
written 22 months ago by
Zhilong Jia • 1.5k
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... 1. try to download it at the deployed website. you will see the downloaded files as follow.
![enter image description here][1]
2. `The expression profile data should be processed data, in which the fold change of one gene between group A and group B could be calculated with ValueA/ValueB`. Actually ...
written 22 months ago by
Zhilong Jia • 1.5k
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2
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5.4k
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... An enhancement : a function to transform the input matrix. ...
written 22 months ago by
Zhilong Jia • 1.5k
0
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1
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640
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1
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Answer:
A: Heatmap2 code error
... Add `Colv=NA` to `heatmap.2(as.matrix(X), col=redgreen(75), scale="row", key=T, keysize=1.5, ` `density.info="none", trace="none",cexCol=0.9, labRow=NA, Colv=NA)`.
For detail, see manual of `gplots::heatmap.2`
BTW, see https://www.biostars.org/p/309884/ to get how to upload figures. `https://i.i ...
written 22 months ago by
Zhilong Jia • 1.5k
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... Powerful. I will recommend to my colleagues. Two small bugs. 1. the try samples cannot be downloaded correctly. 2.heatmap cannot show negative value. BTW, how does it calculate the group expression per gene?
For 1. ![enter image description here][1]
For 2. ![enter image description here][2]
[1 ...
written 22 months ago by
Zhilong Jia • 1.5k
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