User: polys

gravatar for polys
polys0
Reputation:
0
Status:
New User
Last seen:
20 hours ago
Joined:
2 years, 2 months ago
Email:
l**********@gmail.com

Profile information, website and location are not shown for new users.

This helps us discourage the inappropriate use of our site.

Posts by polys

<prev • 7 results • page 1 of 1 • next >
0
votes
1
answer
81
views
1
answers
Comment: C: Bowtie2 MAPQ difference using local vs end-to-end alignment
... Thanks benformatics! Cristal clear ...
written 4 days ago by polys0
0
votes
1
answer
81
views
1
answers
Comment: C: Bowtie2 MAPQ difference using local vs end-to-end alignment
... Thanks for your answer, benformatics. I Will try to repeat the alignment step with Hisat2 just to compare results. So, in your opinion, end to end alignment is the best choice? In which cases would you say it's better to perform local alignment? ...
written 6 days ago by polys0
1
vote
1
answer
81
views
1
answer
Bowtie2 MAPQ difference using local vs end-to-end alignment
... Hello everyone, I am currently trying to learn about ngs data analysis. Specifically, I have data from targeted (panel) ngs from human liquid biopsy samples, in which we have to determine the presence of somatic variants that are at a very low allele fraction (ctDNA in peripheral blood). The compan ...
bowtie2 alignment target ngs written 7 days ago by polys0 • updated 7 days ago by benformatics640
0
votes
1
answer
1.3k
views
1
answers
Comment: C: multiplex primers design software
... thanks! unfortunatedly this soft is not appropiate either. Thanks anyway! ...
written 2.2 years ago by polys0
0
votes
1
answer
1.3k
views
1
answers
Comment: C: multiplex primers design software
... thanks, I've already checked MPprimer as well, no luck either :-( ...
written 2.2 years ago by polys0
0
votes
1
answer
1.3k
views
1
answers
Comment: C: multiplex primers design software
... Hey, thanks for the reply. I've already tried PCRTiler, but it seems to work on only one microorganism at a time, since I cannot select more than one target in the Specificity section. Or am I missing something? ...
written 2.2 years ago by polys0
0
votes
1
answer
1.3k
views
1
answer
multiplex primers design software
... Hi, I'm sort of new to multiplex pcr primers design, and I cannot seem to find a software that suits my needs.... I need to design 3 pairs of primers to detect 3 different microorganisms in one PCR reaction. I was wondering if there's any soft that can: - design 3 pairs of primers compatible with ...
multiplex pcr written 2.2 years ago by polys0 • updated 2.2 years ago by WouterDeCoster37k

Latest awards to polys

Popular Question 2.2 years ago, created a question with more than 1,000 views. For multiplex primers design software

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 2131 users visited in the last hour