User: johannes.berstein

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Posts by johannes.berstein

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Comment: C: qRT-PCR: Data analysis
... So, Ok thanks my reference gene is really reliable and I have few variation... the whole problem is that I cannot apply the normal formulas available because this gene is not expressed in normal cells (only virus infected ones) ...
written 2.9 years ago by johannes.berstein0
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Comment: C: qRT-PCR: Data analysis
... Ok, thanks Any other suggestion? I am wondering if I throw this set of experiments.... :/ ...
written 2.9 years ago by johannes.berstein0
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Comment: C: qRT-PCR: Data analysis
... Ja sure, Just an aditional question: is it acceptable to calculate the fold change like this: 2^(CT target / CT Beta actin). If so this would give us results acceptable results and in accordance to the CT values. ...
written 2.9 years ago by johannes.berstein0
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Comment: C: qRT-PCR: Data analysis
... Hi Absolute quantification is not possible once we dont have a standard curve. As control gene I use beta actin to normalize the genes. Even though the results seems to be quite strange after analysis but our raw data shows clearly a huge difference. :/ The normal methods ddCT or 2^-ddCT seems not ...
written 2.9 years ago by johannes.berstein0
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Comment: C: qRT-PCR: Data analysis
... Hi, Unfortunately it is not a DNA virus. :/ Regards ...
written 2.9 years ago by johannes.berstein0
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qRT-PCR: Data analysis
... Hi everyone, I am trying to analyse data from qRT-PCR. My experimental design was the following: I have two groups of cells (A and B) and those were infected with a virus. What we want to know now is the fold change of B in comparison with A. I believe that a relative quantification using the norma ...
relative quantification qrt-pcr written 2.9 years ago by johannes.berstein0 • updated 2.9 years ago by Devon Ryan93k

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