User: bruseq

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bruseq20
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22 hours ago
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1 year, 10 months ago
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Posts by bruseq

<prev • 23 results • page 1 of 3 • next >
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Remove contamination before assembly
... Hello everyone, I am trying to draft a fungal genome, but the raw sequence R1 and R2 contain contamination, so before doing assembly I mapped Raw reads using bowtie2 against refseq Bacterial db and mitochondrial db, then exact unmapped reads using samtools {the unmapped reads are contamination free ...
genome assembly draft genome written 5 weeks ago by bruseq20 • updated 5 weeks ago by h.mon21k
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Answer: A: Masurca assembly denovo
... Thank you for the reply dear ones, I solved this error. ...
written 5 weeks ago by bruseq20
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Masurca assembly denovo
... Hello everyone I am try assemble a denovo genome using MaSuRCA assembler. but it generate an error: ***Assembly stopped or failed, see CA.log*** Please guide me how to solve this error. Thanks, Divya ...
genome assembly denovo written 5 weeks ago by bruseq20
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Comment: C: How to deal with MSTRG tag without relevant gene name?
... Hello everyone, I also have above same problem i.e; ( in my case Cuffdiff gives gene ID but there specific gene names are missing) I used reference.gtf file during every steps. I also try to get specific gene name using there chr. locus number but no result found, did blast also. No any information ...
written 7 months ago by bruseq20
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Popoolationte Samro.pl error
... Hello everyone, I am using Popoolationte tool. While using tool an error message occur i.e; " sam is not empty in the end at samro.pl line 114, <$fh> line 113468176." while using Samro.pl script for paired-end of Popoolationte. Both the given input sam files are equal in size. I am not get ...
popoolationte samformat written 16 months ago by bruseq20
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Comment: C: Samtools mpileup degenerative characters
... Thanks for the comment @ Saumel That I know the -1 are the indels but I didn't get why these "y" and "s" characters also encounter with -1 in the mpileup file. ...
written 16 months ago by bruseq20
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Comment: C: Samtools mpileup degenerative characters
... Hello sir, Now I installed samtools version-1.5 but the problem is persist in the mpileup file. Any suggestion. ...
written 16 months ago by bruseq20
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Comment: A: Samtools mpileup degenerative characters
... Thanks for your reply, I m using samtools version `0.1.19-96b5f2294a` Here below I m posting the command samtools mpileup -f ref_genome.fa input.bam > output.mpileup Error lines 2 3616324 A 211 .,,.,..,-1y...,..,,..............,,,,,,......,,,...,,,....,,,....,,,,,,,.....,,..,....... ...
written 16 months ago by bruseq20 • updated 16 months ago by Istvan Albert ♦♦ 78k
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Samtools mpileup degenerative characters
... Hello everyone, I am using samtools to get mpileup file. but there is an degenerative characters "-1y, -1Y, -1s" etc. also shown in the mpileup file. I want to know why these degenerative characters are shown in mpileup file. Please help me to sort the above problem. Thanks, Divya ...
samtools degenrative characters mpileup written 16 months ago by bruseq20 • updated 4 months ago by swbarnes24.5k
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Comment: C: Popoolation variance-sliding.pl output shows only zero value
... Please anyone guide me how to solve this error. ...
written 16 months ago by bruseq20

Latest awards to bruseq

Popular Question 4 days ago, created a question with more than 1,000 views. For extract SNP, variants
Popular Question 4 days ago, created a question with more than 1,000 views. For STRUCTURE software file format
Popular Question 7 months ago, created a question with more than 1,000 views. For extract SNP, variants
Popular Question 13 months ago, created a question with more than 1,000 views. For R unused arguments

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