User: Hughie

gravatar for Hughie
Hughie30
Reputation:
30
Status:
New User
Location:
guangzhou
Last seen:
3 weeks, 3 days ago
Joined:
1 year, 1 month ago
Email:
9********@qq.com

A beginner in bioinformatics.

Posts by Hughie

<prev • 26 results • page 1 of 3 • next >
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Comment: C: DESeq result "LFC > 0 (up) : 0, 0% ,LFC > 0 (down) : 0, 0%"
... Thank you again! Santosh. I will try to figure out these packages and the link is very specific. ...
written 5 weeks ago by Hughie30
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Comment: C: DESeq result "LFC > 0 (up) : 0, 0% ,LFC > 0 (down) : 0, 0%"
... Thank you! Santosh. These two pages are very useful and I will try more. BTW,except edgeR, is there any packages can be recommended for DEG analysis. Thank you again for your reply. ...
written 5 weeks ago by Hughie30
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DESeq result "LFC > 0 (up) : 0, 0% ,LFC > 0 (down) : 0, 0%"
... Hello! All. I'm using DESeq to check differential gene expression , but I got in doubt recent days and meet strange result which different from DESeq munual's demo , below I post my code and wish your kindly help: library(DESeq2) workDir <- "C:/Users/Administrator/Desktop/rawcounts" ...
rna-seq written 5 weeks ago by Hughie30
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FetureCounts low assigned rate and its weird total number counts?
... Hi! myfriends, I'm doing read count of my RNA-seq data using featurecounts, I found a pretty low assigned rate which ranges 20~30%, like below. FeatureCounts result I noticed that the total is not accord with my FastQC and STAR' report: So, I used samtools flagstat to check: I'm in ...
next-gen rna-seq written 6 weeks ago by Hughie30
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RSeQC geneBody_coverage and RPKM_saturation result exlaination
... Hello! Recently I'm doing post mapping quality check using RSeQC. I mainly checked geneBody_coverage and RPKM_saturation using its python module, but I don't know what's good and bad result, so I post my result here and wish your help. ![geneBody_coverage][1] I can't explain the result, I guess ...
genome alignment next-gen rna-seq sequencing written 5 months ago by Hughie30
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Comment: C: FastQC's report -- 'Per base sequence content' heading odd wave
... Thank you James! I will check the kit and find out the reason. ...
written 6 months ago by Hughie30
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Comment: C: FastQC's report -- 'Per base sequence content' heading odd wave
... Thank you lelle! So do you think it's necessary to trim the header 10 bases? ...
written 6 months ago by Hughie30
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Comment: C: FastQC's report -- 'Per base sequence content' heading odd wave
... Thank you genomax! You are always so kindly! The additional information is so useful, Thank you again. ...
written 6 months ago by Hughie30
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FastQC's report -- 'Per base sequence content' heading odd wave
... Hello! I'm a beginner of bioinformatics and recently I got some FastQC report when QC my RNA-seq data, but I can't understand the odd situations showing below. https://ibb.co/caxZEv https://ibb.co/hU7ZEv https://ibb.co/cnSZEv I want to know what caused the heading odd waves and how to handle th ...
rna-seq written 6 months ago by Hughie30 • updated 6 months ago by GenoMax42k
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Comment: C: How to identify your analytic data is true?
... Thank you Michael for your kindly behavior! I meant closer to "measurement error", and do you know some bioinformatic methods to judge? Thank you again. ...
written 6 months ago by Hughie30

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