User: Hughie

gravatar for Hughie
Hughie30
Reputation:
30
Status:
New User
Location:
guangzhou
Last seen:
4 months, 3 weeks ago
Joined:
1 year, 5 months ago
Email:
9********@qq.com

A beginner in bioinformatics.

Posts by Hughie

<prev • 26 results • page 1 of 3 • next >
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Comment: C: DESeq result "LFC > 0 (up) : 0, 0% ,LFC > 0 (down) : 0, 0%"
... Thank you again! Santosh. I will try to figure out these packages and the link is very specific. ...
written 5 months ago by Hughie30
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Comment: C: DESeq result "LFC > 0 (up) : 0, 0% ,LFC > 0 (down) : 0, 0%"
... Thank you! Santosh. These two pages are very useful and I will try more. BTW,except edgeR, is there any packages can be recommended for DEG analysis. Thank you again for your reply. ...
written 5 months ago by Hughie30
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DESeq result "LFC > 0 (up) : 0, 0% ,LFC > 0 (down) : 0, 0%"
... Hello! All. I'm using DESeq to check differential gene expression , but I got in doubt recent days and meet strange result which different from DESeq munual's demo , below I post my code and wish your kindly help: library(DESeq2) workDir <- "C:/Users/Administrator/Desktop/rawcounts" ...
rna-seq written 5 months ago by Hughie30
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FetureCounts low assigned rate and its weird total number counts?
... Hi! myfriends, I'm doing read count of my RNA-seq data using featurecounts, I found a pretty low assigned rate which ranges 20~30%, like below. FeatureCounts result I noticed that the total is not accord with my FastQC and STAR' report: So, I used samtools flagstat to check: I'm in ...
next-gen rna-seq written 5 months ago by Hughie30
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RSeQC geneBody_coverage and RPKM_saturation result exlaination
... Hello! Recently I'm doing post mapping quality check using RSeQC. I mainly checked geneBody_coverage and RPKM_saturation using its python module, but I don't know what's good and bad result, so I post my result here and wish your help. ![geneBody_coverage][1] I can't explain the result, I guess ...
genome alignment next-gen rna-seq sequencing written 9 months ago by Hughie30
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Comment: C: FastQC's report -- 'Per base sequence content' heading odd wave
... Thank you James! I will check the kit and find out the reason. ...
written 10 months ago by Hughie30
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Comment: C: FastQC's report -- 'Per base sequence content' heading odd wave
... Thank you lelle! So do you think it's necessary to trim the header 10 bases? ...
written 10 months ago by Hughie30
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Comment: C: FastQC's report -- 'Per base sequence content' heading odd wave
... Thank you genomax! You are always so kindly! The additional information is so useful, Thank you again. ...
written 10 months ago by Hughie30
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FastQC's report -- 'Per base sequence content' heading odd wave
... Hello! I'm a beginner of bioinformatics and recently I got some FastQC report when QC my RNA-seq data, but I can't understand the odd situations showing below. https://ibb.co/caxZEv https://ibb.co/hU7ZEv https://ibb.co/cnSZEv I want to know what caused the heading odd waves and how to handle th ...
rna-seq written 10 months ago by Hughie30 • updated 10 months ago by genomax49k
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Comment: C: How to identify your analytic data is true?
... Thank you Michael for your kindly behavior! I meant closer to "measurement error", and do you know some bioinformatic methods to judge? Thank you again. ...
written 10 months ago by Hughie30

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