User: Titus

gravatar for Titus
Titus400
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400
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4 hours ago
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7 months, 1 week ago
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Posts by Titus

<prev • 113 results • page 1 of 12 • next >
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Comment: C: Looking for wig base coverage file for hg19
... I didn't get it but i didn't try for a month .... ...
written 4 days ago by Titus400
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Comment: C: how to get exact break point in COSMIC fusion gene?
... I think you have to translate it ... I had the same problems in my lab because some people use only amino acid mutation ... I don t know software whitch reverse that , may be with some "home made solution" using annovar , snpEFF or VEP ? ...
written 5 days ago by Titus400
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Comment: C: Locate short DNA sequence from fasta file
... So you should to post the answer for the other ! ...
written 8 days ago by Titus400
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Comment: C: BRCA1 and BRCA2 database's for NGS diagnostics purposes
... Hi you can have a look here : http://www.umd.be/ . The advantage is a manual curated database by biologist. ...
written 8 days ago by Titus400
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Comment: C: How to reduce cross mapping alignments with paired-end reads ?
... Well it make sense if in your genome you have only this gene covered , depends on the genome size so in % coverage etc ... I thinks genome lengths bacterial are available (for the most popular). If you have fasta you can simply calculate the length :) ...
written 12 days ago by Titus400
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Comment: C: How to reduce false positive whan aligning paired-end reads ?
... Yes there is plenty of solution : - You can make a filter on the % of coverage of your genomes ( in your case you will get only this gene covered on your Species B reference isn't it ? ) - You can select for each ref of your species only specific part etc ... The solution will depends on what ...
written 12 days ago by Titus400
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Comment: C: How to reduce false positive whan aligning paired-end reads ?
... Did you check if your pairs map on the same ref ? ...
written 12 days ago by Titus400
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Comment: C: How to reduce false positive whan aligning paired-end reads ?
... Agree with ATPoint , why do you align against Species B ref if it's not possible to find it ? You can still change your parameters to filter reads with Mismatch penalty , but you will got less aligned reads in global mapping. ...
written 12 days ago by Titus400
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Comment: C: Which tool is recommended for detecting CNVs in whole exome sequencing?
... I add XHMM (http://atgu.mgh.harvard.edu/xhmm/tutorial.shtml ) ...
written 12 days ago by Titus400
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Comment: C: Aligning short sequences to fastq
... Hi, May be you can try to ta align with bwa aln your 23 bps seq against your fastq files as ref after you transformed it as fasta ? Best ...
written 15 days ago by Titus400

Latest awards to Titus

Commentator 7 days ago, created a comment with at least 3 up-votes. For C: BWA: Why paired reads mapped to different chromosome?
Centurion 19 days ago, created 100 posts.
Supporter 8 weeks ago, voted at least 25 times.
Appreciated 4 months ago, created a post with more than 5 votes. For C: BWA: Why paired reads mapped to different chromosome?
Commentator 4 months ago, created a comment with at least 3 up-votes. For C: BWA: Why paired reads mapped to different chromosome?
Rising Star 4 months ago, created 50 posts within first three months of joining.
Scholar 5 months ago, created an answer that has been accepted. For C: finding homologs in human
Teacher 5 months ago, created an answer with at least 3 up-votes. For C: where to find a list of often mutated or lost regions in cancers
Scholar 5 months ago, created an answer that has been accepted. For C: finding homologs in human

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