User: Titus

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Titus440
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Posts by Titus

<prev • 136 results • page 1 of 14 • next >
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Comment: C: Inconsistent Coverage on X chromosome between genders
... You can add this condition : during amplification if the PCR cycle are under 35 cycles you can be quantitative for X ...
written 1 hour ago by Titus440
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Comment: C: Inconsistent Coverage on X chromosome between genders
... Thank you for the reply. I discuss with so colleagues in the lab , they explained to me that during amplification if the PCR cycle are under 35 cycles you can be quantitative for X. That's make sens too. ...
written 1 hour ago by Titus440
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Comment: C: Inconsistent Coverage on X chromosome between genders
... Thank's to take the time to check that bias, and give me a feed back if you have one :) ...
written 2 hours ago by Titus440
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Comment: C: Inconsistent Coverage on X chromosome between genders
... i think during the capture your DNA is in saturation compare of your probes, so at he end you have the same quantity of DNA amplified. That confirmed by the ration between homozygous and heterozygous SNP you should get. So i have wrong theory but i don't understand why there is this ratio .... ...
written 1 day ago by Titus440
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Comment: C: Inconsistent Coverage on X chromosome between genders
... Well don't you think enrichment / selected region saturation (during library preparation) is the main reason ? ...
written 1 day ago by Titus440
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Comment: C: Inconsistent Coverage on X chromosome between genders
... I m not sure to understand what you mean about target and off target. For the problem if i understand correctly is that you don't have the ratio between male an female you expected right ? To me ( may be i m wrong ) to get this ratio you should check heterozygous and homozygous SNP , because i don ...
written 1 day ago by Titus440
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Comment: C: visualization of identified SSRs
... You should precise your output file format ...
written 1 day ago by Titus440
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Comment: C: De novo sequencing?
... The point is there is structural variation in genome between organism from the same species , so you can speak about De novo sequencing for every organism to my point of view ! If you check all genome reference organism have N , so genomes are partially sequenced because of the limits of assembly l ...
written 1 day ago by Titus440
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Comment: C: Exon coordinates and sequence
... You can extract coordinates of exon from ucsc (https://genome.ucsc.edu/cgi-bin/hgTables ) to be sure you compare the same ENST ...
written 2 days ago by Titus440
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Comment: C: Exon coordinates and sequence
... Hi where you gtf file come from ? There is few possibility for this error like not the same NM gene/transcript version so the coordinates from gtf files and ucsc are not the same. ...
written 2 days ago by Titus440

Latest awards to Titus

Commentator 14 days ago, created a comment with at least 3 up-votes. For C: BWA: Why paired reads mapped to different chromosome?
Commentator 10 weeks ago, created a comment with at least 3 up-votes. For C: BWA: Why paired reads mapped to different chromosome?
Centurion 12 weeks ago, created 100 posts.
Supporter 4 months ago, voted at least 25 times.
Appreciated 6 months ago, created a post with more than 5 votes. For C: BWA: Why paired reads mapped to different chromosome?
Commentator 6 months ago, created a comment with at least 3 up-votes. For C: BWA: Why paired reads mapped to different chromosome?
Rising Star 6 months ago, created 50 posts within first three months of joining.
Scholar 7 months ago, created an answer that has been accepted. For C: finding homologs in human
Teacher 7 months ago, created an answer with at least 3 up-votes. For C: where to find a list of often mutated or lost regions in cancers
Scholar 8 months ago, created an answer that has been accepted. For C: finding homologs in human

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