User: xzpgocxx

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xzpgocxx0
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Posts by xzpgocxx

<prev • 13 results • page 1 of 2 • next >
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Comment: C: Bionano assembly genome have too much N
... Actually, I have an email to Bionano support, but the reply is confusing. > It might not be clear why i referred you to the xml as this contains descriptions that might help you to improve as all parameters are described there. How ever bionano has put a lot of effort in getting the parameter ...
written 3 days ago by xzpgocxx0
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Bionano assembly genome have too much N
... Hi, I want to assemble my study genome use PacBio long sequence and Bionano with Hybrid-Scaffold way. Firstly, I assemble the contigs use PacBio long sequence and the resulted have only 0.1% N. Then, I assemble the scaffolds use Bionano data (Direct Label and Stain (DLS) chemistry ) as the sugg ...
genome assembly sequencing written 4 days ago by xzpgocxx0
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short sequence alignment to short sequence
... Hi, I have many short sequences (60bp) and want to alignment to the short reference sequence (also is 60bp) to get the different alignment types. I have to try blast to do this work and output m8 format to get the mismatches and gap value. But It's not enough and I'm interested abundant information ...
sequence alignment written 9 weeks ago by xzpgocxx0
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Statistical the DNA mutation types
... Hi, I want to statistical the mutation types based on sequence alignment. Besides, I'm only interested in the mutation present in the red frame[1] (The first sequence is the reference sequence). I have a huge amount of fasta sequences and cannot be count by the manual. So, is there any software o ...
sequence alignment written 9 weeks ago by xzpgocxx0
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How to assess a genome is appropriate to do BioNano?
... Hi, We have DeNovo assemble the genome used PacBio data. Now, we want to improve the N50 through BioNano. My question is how to assess my study genome is appropriate to do the BioNano? Thanks. Best, ...
genome assembly written 11 weeks ago by xzpgocxx0
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Comment: C: How to calculate the different expression gene used PacBio full-length cDNA seq
... Thanks, It's really the best guide to solve this question. Let me try. ...
written 8 months ago by xzpgocxx0
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Comment: C: BLESS : ERROR: Irregular quality score range 35-75
... bless -read1 3k_R1.rd.clean.fastq -read2 3k_R2.rd.clean.fastq -prefix ../bless/3k_R12.rd.clean -kmerlength 31 ...
written 10 months ago by xzpgocxx0
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Comment: C: BLESS : ERROR: Irregular quality score range 35-75
... Yes, command-line, VERSION: 1.02 ...
written 10 months ago by xzpgocxx0
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BLESS : ERROR: Irregular quality score range 35-75
... I get follows error output when used the [bless][1] to correct my Illumina paired-end reads, which I have trimmed used Trimmomatic software. Checking input read files ERROR: Irregular quality score range 35-75 -------------------------------------------------------------------------- ...
software error next-gen written 10 months ago by xzpgocxx0
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How to calculate the different expression gene used PacBio full-length cDNA sequencing and Illumina sequencing?
... Now, I have some data from PacBio full-length cDNA sequencing and Illumina sequencing. And I want to calculate the gene expression leave (FPKM). There is no available genome for my species. I can do it used Trinity if only have Illumina data, but now I don't know how to do it when adding PacBio fu ...
rna-seq written 12 months ago by xzpgocxx0 • updated 8 months ago by tjduncan170

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