User: kmurph55

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kmurph5510
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2 years, 11 months ago
Joined:
3 years, 3 months ago
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k*******@montgomerycollege.edu

Posts by kmurph55

<prev • 6 results • page 1 of 1 • next >
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Comment: C: What could be wrong with my FASTQ files? Picard suggests that there is missing h
... Thanks!! ... I didnt realize that this information needed to be set by the user. ...
written 3.3 years ago by kmurph5510
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Comment: C: What could be wrong with my FASTQ files? Picard suggests that there is missing h
... Yes this was just an error that I made in my post. ...
written 3.3 years ago by kmurph5510
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What could be wrong with my FASTQ files? Picard suggests that there is missing header information.
... Hello, I have two fastq files 3D_1.fastq and 3d_2.fastq. To the best of my knowledge the first file contains forward reads and the second file contains reverse reads. I am able to confirm that the fastq files were generated as paired end reads, 101 base pairs in length, and have Illumina/sanger 1.9+ ...
software error sequencing written 3.3 years ago by kmurph5510 • updated 3.3 years ago by Santosh Anand5.1k
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Comment: C: HiSeq2500, Whole Genome Resequencing, Paired-ends 101 bp length, but no barcode
... I have been researching different pipelines for handling fastq files and almost everything that I have read suggests preprocessing the fastq data prior to mapping the reads via bowtie2 or BWA etc. So by "joining the reads into mate pairs" I meant that my fastq files are currently separate files wher ...
written 3.3 years ago by kmurph5510
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Answer: A: HiSeq2500, Whole Genome Resequencing, Paired-ends 101 bp length, but no barcode
... Thank you for the quick reply. The main problem that I am facing is that the Read Group identifier is supposed to be unique to each read for most NGS tools, but every read group ID is the exact same (with the exception of my x and y coordinates) in my fastq files. ...
written 3.3 years ago by kmurph5510
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HiSeq2500, Whole Genome Resequencing, Paired-ends 101 bp length, but no barcode! Please Help
... I have two separate fastq files, one file for each mate of a pair. Each file has a corresponding .txt file, but only offers a summary of quality information. I believe my paired-end reads are multiplexed, but I have no way of identifying them on a unique basis. From my understanding I am supposed to ...
sequencing written 3.3 years ago by kmurph5510

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