User: realnewbie

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realnewbie10
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Posts by realnewbie

<prev • 19 results • page 1 of 2 • next >
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Answer: A: how to fix Kmer count from FastQC?
... Is it because of adapter content? If it is so, there are some tools that might help you in the adapter trimming process. ...
written 2 days ago by realnewbie10
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Comment: C: NextSeq 500 RNA-Seq results
... You are god damn right :) Actually, I am trying very hard. I did not take any course that teaches RNA-seq data analysis from scratch. I am trying to understand and learn every detail in a very short period of time . To combine all the details together and produce something meaningful and right could ...
written 3 days ago by realnewbie10
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Comment: C: Multiple Conditions Testing in DESeq2 with unequal number of replicates
... Yes,you are right. Thanks for your time. However, I was wrong. Sorry for misleading to you. Each treatment is different from each other (but the .1 or.2 meaning relicas for same treatment conditions from different wells),so each control of each treatment is also different(i.e: targeting vector vs em ...
written 3 days ago by realnewbie10
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Comment: C: Multiple Conditions Testing in DESeq2 with unequal number of replicates
... same cell lines with same treatment, but from different wells. ...
written 4 days ago by realnewbie10
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Answer: A: Technical and Biological replicates
... Technical and Biological Replicates clearly explained [here][1]. -Yes,induuude [1]: https://www.youtube.com/watch?v=Exk0OoRG0PQ ...
written 4 days ago by realnewbie10
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Multiple Conditions Testing in DESeq2 with unequal number of replicates
... Hi, I read previous posts about multiple conditions testing. However, my case is a little bit different. I have 3 different groups (plus their controls) with **unequal number of technical replicates**, so I have trouble to how to compare them. First I want to compare each treated sample with its ...
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Comment: C: Tutorials for Library Normalization in DESeq2 and EdgeR
... He is awesome in explaining all of these interesting concepts. ...
written 4 days ago by realnewbie10
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Comment: C: GeneReader RNA-Seq results
... Actually, I haven't used raw sequencing data (fastq files). I used bam files and continue with them through HT-seq. HT-seq gave me the raw counts (for annoatation I used gencode.v19.annotation.gft , the others from ensembl did not work. I dont know why they did not work). ...
written 4 days ago by realnewbie10
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Comment: C: GeneReader RNA-Seq results
... totally, agree. It also causes a trouble for newbies like me (I was searching about txt.gz format). ...
written 4 days ago by realnewbie10
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Comment: C: GeneReader RNA-Seq results
... I was thinking that is a manual given with rna-seq results :') I was thinking too complex. Thanks a lot! By the way, I learnt that **txt.gz** is a compressed version of **fastq** files. ...
written 4 days ago by realnewbie10

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