User: netsam

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netsam0
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Posts by netsam

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Comment: C: Little help for paper translation
... That's ok, I agree with you. I'm sorry for the situation. ...
written 6 days ago by netsam0
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Comment: C: Little help for paper translation
... Grazie, si, ho scritto il lavoro direttamente in inglese e vorrei capire se ci sono errori grammaticali e, soprattutto, se il senso della frase è giusto. Ma penso che sarà troppo lungo fare la correzione in un post. Mi potresti aiutare via mail ? ...
written 6 days ago by netsam0
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Comment: C: Little help for paper translation
... Ok, thanks for your tips. I'll check the attached documents. Sorry but I have to connect this sentence with next. I wrote the three sentences together. The aim of this study was to investigate the frequency of the alleles of 10 microsatellite markers in Sicilian sheep, providing a first reference ...
written 8 days ago by netsam0
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Comment: C: Little help for paper translation
... Sorry, I'm from Italy but the paper is written in English. "The aim of this study was to investigate allelic frequencies in 10 of the microsatellite markers recommended by the International Society for Animal Genetics (ISAG) in sheep" This is part of Abstract. The data have been produced by fragme ...
written 8 days ago by netsam0
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(Closed) Little help for paper translation
... Hi, I would ask some little help for the translation to english of an article on microsatellite analysis . I'm not native English. I do not even know if it's possible in this forum ask this type of question. ...
sequencing written 8 days ago by netsam0
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Comment: C: Assembly NGS Reads from D-LOOP Wolf
... Thanks, I appreciate your help. ...
written 10 days ago by netsam0
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Comment: C: Assembly NGS Reads from D-LOOP Wolf
... I changed my approach, - Mapped my reads with Bowtie against complete Mitochondrial DNA of Wolf (~16.000 bp) - Extract mapped reads (~2.000 reads) - Assemble contig of mtDNA of sample with Spades and trusted contig (same mtDNA for mapping) - Search DLOOP sequence inside my assembled contig with Emb ...
written 10 days ago by netsam0
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Comment: C: Assembly NGS Reads from D-LOOP Wolf
... yes, it matches perfectly 100% with a mitochondrial region between 11201 and 11593. I think that that isn't D-LOOP region. In a sequence that I extracted from reads of another sample, there was a perfect match with D-LOOP sequence in a region of mitochondrion starting from around 16000 bp. Because D ...
written 10 days ago by netsam0
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Comment: C: Assembly NGS Reads from D-LOOP Wolf
... With blast there's a match with Canis lupus isolate Mongolia2 mitochondrion, partial genome, and other similar sequence; NOT d-loop. I'll try to visualize with IGV. I mapped reads with Bowtie 2, maybe it's a method, algorithm or parameters problem? ...
written 10 days ago by netsam0
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Comment: C: Assembly NGS Reads from D-LOOP Wolf
... Ok, I extract the reads mapping against a D-LOOP of almost 450 bp and I obtain 100 sequence paired end (50R + 50L ). If I multiple 50 * 100 bp (average length of a reads) I get 5.000 bp. 5.000 bp / 450 = almost 10 x This is the coverage of reads ? And what is the coverage of contigs ? ...
written 12 days ago by netsam0

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