User: Alexie Li
Alexie Li • 20
- Reputation:
- 20
- Status:
- New User
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- Last seen:
- 14 minutes ago
- Joined:
- 2 months, 3 weeks ago
- Email:
- y********@gmail.com
Posts by Alexie Li
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... Hi all!
I have a question about is there a particular requirement for RNA-seq to study poly(A) in a species?
I have many rna-seq data using poly(A) based library preparation method, but they are not designed to study alternative polyadenylation problem. From my understanding, the data should harbor ...
written 8 days ago by
Alexie Li • 20
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... I think maybe I understand what is happening with the uneven expression. FastQC showed enriched clusters at the beginning of reads which means the library is biased by random priming during library preparation. Maybe it is why...
...
written 6 weeks ago by
Alexie Li • 20
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... Dear all,
I have a confusion about antisense detection. From my RNA-seq result which is strand-specific and polyA enriched during library preparation, about one-third of genes showed antisense expression. Does it mean all the antisenses detected here have polyA tails/polyadenylated?
Thank you for y ...
written 6 weeks ago by
Alexie Li • 20
• updated
6 weeks ago by
chalchiuhticuenaabah • 0
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... Hi all,
I have a question about gene expression. I mapped RNA-seq reads back to the genome and noticed there are my expression peaks within a gene. Does it cause by library preparation process, sequencing problem, or a real biological thing?
The blue bar on the top is a gene(single-exon gene), the r ...
written 11 weeks ago by
Alexie Li • 20
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... It worked! Thank you. ...
written 11 weeks ago by
Alexie Li • 20
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315
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... Hi russhh!
Thank you for your help.
I tried this method but failed to get the result, I think there are two problems
1)I can't sort file 1 since I need the order information
2)For some reason, my system is not recognizing "join -t $'\t'" and gave the error message "join: illegal tab character spec ...
written 11 weeks ago by
Alexie Li • 20
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6 follow
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... Hi all
I am too new with programing to solve this problem. I have two files, file1 containing the index, file2 includes information I want.
1. file1
CU_91
CU_495
CW_79
CU_22
CW_42
2. file2
CW_79 protein1
CW_15 protein ...
written 11 weeks ago by
Alexie Li • 20
• updated
11 weeks ago by
Pierre Lindenbaum ♦ 99k
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... Actually, they are a merged file of results generated by different methods, I want to check which method generally give the best results or more reliable. ...
written 11 weeks ago by
Alexie Li • 20
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... Hi all,
I have a file containing blast hits for each gene, one hit per line, as following
ID E-value
g1 0.0
g1 1e-20
g1 1e-5
g2 1e-5
g2 1e-13
g3 0.01
I would like to grep the lowest e-value for each gene, how to write a script to make it? ...
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5 follow
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... Hi all,
I got a file with the first column containing id and second column containing annotated gene ontology numbers. As the following
CPIW_00004002-RA GO:0005515
CPIW_00004002-RA GO:0010997|GO:0097027|GO:1904668
CPIW_00004003-RA GO:0003824|GO:0008152
CPIW_00004003-RA GO:0003987|GO:0016208|GO ...
written 12 weeks ago by
Alexie Li • 20
• updated
12 weeks ago by
Chirag Parsania • 430
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