User: Alexie Li
Alexie Li • 20
- Reputation:
- 20
- Status:
- New User
- Location:
- Last seen:
- 2 years, 5 months ago
- Joined:
- 3 years, 7 months ago
- Email:
- y********@gmail.com
Posts by Alexie Li
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• 13 results •
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... I am using WGCNA to generate gene co-expression network. I am confused about the how to interpret gene module colors. My understanding is, different modules will have different color. So the downstream analysis could be based a specific colored module such as blue. However, the cluster dendrogram fi ...
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Horizontal axis indicate features on chromosomes. Y axis displays multiple chromosomes.
Thank you!
Best wishes,
Alexie Li ...
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... Hi all,
I have alignment five close-related genomes. A, B, C, D and E. A and B share nucleotide level identity as 96%, while between A and C this number is 94%. I used several methods to build their phylogeny trees. All the result showed A is closer to C and B. I am curious why. Shouldn't the genom ...
written 3.1 years ago by
Alexie Li • 20
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... Hi all!
I have a question about is there a particular requirement for RNA-seq to study poly(A) in a species?
I have many rna-seq data using poly(A) based library preparation method, but they are not designed to study alternative polyadenylation problem. From my understanding, the data should harbor ...
written 3.4 years ago by
Alexie Li • 20
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... I think maybe I understand what is happening with the uneven expression. FastQC showed enriched clusters at the beginning of reads which means the library is biased by random priming during library preparation. Maybe it is why...
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written 3.5 years ago by
Alexie Li • 20
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... Dear all,
I have a confusion about antisense detection. From my RNA-seq result which is strand-specific and polyA enriched during library preparation, about one-third of genes showed antisense expression. Does it mean all the antisenses detected here have polyA tails/polyadenylated?
Thank you for y ...
written 3.5 years ago by
Alexie Li • 20
• updated
3.5 years ago by
chalchiuhticuenaabah • 0
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... Hi all,
I have a question about gene expression. I mapped RNA-seq reads back to the genome and noticed there are my expression peaks within a gene. Does it cause by library preparation process, sequencing problem, or a real biological thing?
The blue bar on the top is a gene(single-exon gene), the r ...
written 3.6 years ago by
Alexie Li • 20
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... It worked! Thank you. ...
written 3.6 years ago by
Alexie Li • 20
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... Hi russhh!
Thank you for your help.
I tried this method but failed to get the result, I think there are two problems
1)I can't sort file 1 since I need the order information
2)For some reason, my system is not recognizing "join -t $'\t'" and gave the error message "join: illegal tab character spec ...
written 3.6 years ago by
Alexie Li • 20
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... Hi all
I am too new with programing to solve this problem. I have two files, file1 containing the index, file2 includes information I want.
1. file1
CU_91
CU_495
CW_79
CU_22
CW_42
2. file2
CW_79 protein1
CW_15 protein ...
written 3.6 years ago by
Alexie Li • 20
• updated
3.6 years ago by
Pierre Lindenbaum ♦ 134k
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