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Posts by gingergeiger22
... Hi, I am trying to run BWA using the following command: module load bwa/0.7.15 time bwa mem -aCMP -t 12 -R '@RG\tID:MiSeq\tSM:VA-002\tPL:illumina\tLB:XT40 PU:Batch22' referencebasename R1_001.fastq.gz R2_001.fastq.gz > VA-002-aligned_reads.sam Error is: [E::bwa_idx ...
... Hello, We have been using the NEB Next enzyme suite to build libraries for sequencing on our MiSeq. The protocol adds adapters by ligation (a TA-like method I believe) and then you build onto that the indexes in a final PCR. Is it possible to simply design adapters with indexes so that a sequencin ...
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