User: maleknias

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maleknias30
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Posts by maleknias

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Tutorial: One of the easy way to achieve read count and RPKM file
... 1- You can go to web: https://jhubiostatistics.shinyapps.io/recount/ 2- Type your study ID in search box, like; SRP066834. 3- Based on any biological requirement like gene, exon, junction or phenotype, you can download read count table, RSE (RData file) and link to other information. 4- If you wa ...
tutorial rna-seq written 9 days ago by maleknias30 • updated 9 days ago by WouterDeCoster20k
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Comment: C: calculate RPKM from read count and gff by galaxy
... thanks a lot. It seems cool. could you guide me "how I can make .count file" ?? Is this make by "Count.seqs" and "mothur_toolsuit" ? ...
written 10 days ago by maleknias30
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Comment: C: calculate RPKM from read count and gff by galaxy
... Thanks but it's not useful for me. I want to save time and because sample size is big i want use table of read-count. so do it again from the beginning is not cost effective!! ...
written 13 days ago by maleknias30
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calculate RPKM from read count and gff by galaxy
... Hi ! I have a table (58037×117) contain scaled read count for 117 sample (case and control) and gff and gtf file. Considering of RPKM calculation: RPKM=(row number of reads/exon length) ×(1000 000 / number of mapped reads in the sample) how can I get a table (58037×117) contain RPKM?? Is it solutio ...
rna-seq written 13 days ago by maleknias30 • updated 12 days ago by EagleEye4.6k

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