User: koushikponnanna

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Posts by koushikponnanna

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Comment: C: how to signify experiments/replica files in kissplice
... Hi Vincent , Is it advised to merge paired end read files of a sample to one fastq file and further feed it for kissplice analysis? Cos when 2 read files of a sample are treated as 2 samples it gives allele frequency separately. How to resolve this ? And it would be clear if further detailed inform ...
written 8 weeks ago by koushikponnanna0
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Comment: C: Variant detection in RNA seq datasets without a reference using kISSPLICE.
... I am aware of the well characterized reference for few species. But the problem is those are quiet diverged, and using them to call variants would not serve my purpose. That is the reason I am looking to call variants from raw reads. ...
written 8 weeks ago by koushikponnanna0
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Variant detection in RNA seq datasets without a reference using kISSPLICE.
... I am presently working on RNA seq paired end data from few Drosophila tissues. As I dont have an established genome reference I need to rely on reference free variant calling tools and I find KISSPLICE suitable. I have outputs generated for my datasets. I have few questions related to the outputs ge ...
de novo rna-seq snp kissplice written 8 weeks ago by koushikponnanna0 • updated 2 days ago by vincent.lacroix50

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