User: nancy

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nancy70
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Posts by nancy

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single cell RNA-seq
... In scRNA-seq -how does one tell the difference between cells which are in a different temporal phase than other cells hence have a different gene-expression signature, versus actually different cell-type? Is there any way of ensuring a cell population is synchronous before it is subjected to single- ...
single-cell scrna-seq rna-seq written 18 days ago by nancy70 • updated 17 days ago by jared.andrews074.1k
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Answer: A: Gene list of Neuronal Markers for TSNE plot
... Ben Barres lab has made some data publicly available (can dowload an excel spreadsheet with fpkms) here, as well as look up genes enriched in each cell type. https://web.stanford.edu/group/barres_lab/brain_rnaseq.html ...
written 9 months ago by nancy70
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Transforming RNA-seq data- (tpms) - what is the best practice for dealing with zeros for the purpose of deconvolution?
... Using Cibersort to deconvolve bulk RNA-seq data to specific cell-types based on expression values from bulk RNA-seq. I have a series of samples, and their individual TPMs as computed by Stringtie. Each column is a sample, each row is a gene. Since cibersort only accepts non-zero values, I am trying ...
deconvolution cibersort tpm rna-seq written 11 months ago by nancy70
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RNA-seq data analysis for differential expression of a single gene
... I have some RNA-seq data for a large number of samples (treated vs control), to the order of ~50M PE reads per sample, from mouse. These data have not been aligned yet to the reference genome. I am, however interested in the expression level of one single target gene across all these samples, and a ...
rna-seq written 12 months ago by nancy70 • updated 12 months ago by JC9.1k
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Are there minimum requirements from a de novo genome assembly for it to be suitably used as a reference for SNP calling?
... To what extent does a de novo genome need to be assembled for it to be used as a reference? If I have a de novo assembly for a large mammalian genome comprising of a under 1M contigs (Illumina + PacBio), and I want to call SNPs against this reference from samples that have been sequenced (shallow 5 ...
assembly reference genome snp wgs written 16 months ago by nancy70
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RNA-seq data analysis to detect dosage effect or proportional increase/decrease in expression across a series
... Hi- I have some RNA-seq data from a series of 4 isogenic strains (0,1,2,3), differing in their copy number of a single gene. I would now like to identify those genes whose expression changes across these samples in proportion to the copy number of the gene of interest. For e.g., in the series 0,1, ...
dosage effect deseq2 rna-seq written 17 months ago by nancy70 • updated 16 months ago by Biostar ♦♦ 20
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Comment: C: How to filter for samples that show good concordance across replicates in DESEQ2
... Thanks for your detailed and well-explained response, I actually understood it and it makes sense to me now. And thanks for cleaning up my original post too. I learned something there too! ...
written 18 months ago by nancy70
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Comment: C: How to filter for samples that show good concordance across replicates in DESEQ2
... Hi WouterDeCoster, Thanks for showing me how to add figures to the main post. Very useful! I quite disagree with as extreme a view as "any comparison with n=2 entirely invalid and all time you spend is wasted". Often, we do an exploratory n=2 to figure out things like most appropriate timepoint, ...
written 18 months ago by nancy70
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Comment: C: How to filter for samples that show good concordance across replicates in DESEQ2
... Thanks Kevin, 1. No, I do not believe every gene to go down in treated, sorry I grabbed two different sets of data (one from the UP sheet and one from the DOWN sheet) as representative examples of high variance vs good replicates. 2. I didn't generate Cooks Distance- have only 2 reps? but let me t ...
written 18 months ago by nancy70 • updated 18 months ago by zx87548.7k
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Comment: C: hclust heatmap clustering produce different relationships with fpkm versus rlog
... Hi Kevin, Thank you for your detailed reply. It will take me a while to assimilate and understand in as great detail. Having been on the lab side for several years, managing an NGS core lab - the only thing that strikes to me as odd is this part "NGS, samples are always sequenced to different depth ...
written 18 months ago by nancy70

Latest awards to nancy

Popular Question 5 weeks ago, created a question with more than 1,000 views. For stringtie + ballgown for multivariate analysis of rna-seq data
Popular Question 6 months ago, created a question with more than 1,000 views. For stringtie + ballgown for multivariate analysis of rna-seq data
Scholar 9 months ago, created an answer that has been accepted. For A: 6-bp deletions in RNA-seq data
Scholar 20 months ago, created an answer that has been accepted. For A: 6-bp deletions in RNA-seq data

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