User: Ambika

gravatar for Ambika
Ambika20
Reputation:
20
Status:
New User
Location:
United States/Auburn/Auburn University
Last seen:
3 months ago
Joined:
1 year ago
Email:
a***************@gmail.com

Posts by Ambika

<prev • 87 results • page 1 of 9 • next >
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Comment: C: qPCR: Huge variation in fold change of genes between biological replicates
... @Kevin I am just using cDNA to amplify the genes. I did not apply any whole genome amplification ...
written 3 months ago by Ambika20
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Comment: C: qPCR: Huge variation in fold change of genes between biological replicates
... @Kevin Yes I am using ∆∆Ct method to determine the fold change. I collected all my biological samples the same day in order to minimize the variation, and of course the treatments and conditions are same. Even my technical replicates look fine. So, I am having hard time figuring out what is the actu ...
written 3 months ago by Ambika20
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Comment: C: qPCR: Huge variation in fold change of genes between biological replicates
... i already checked the degradation by running gel on my RNA samples before and after DNAse treatment. They look fine. ...
written 3 months ago by Ambika20
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qPCR: Huge variation in fold change of genes between biological replicates
... Hello everyone, I am trying to validate my RNAseq data by doing qpcr for which I am looking at the fold change of few genes across various timepoints of treatment conditions. I am getting huge amount of variation (in thousand folds ) in my biological replicates. I thought may be it is due to genomi ...
foldchange qpcr written 3 months ago by Ambika20 • updated 3 months ago by WouterDeCoster32k
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Comment: C: Gene enrichment analysis
... Thank you I will try that ...
written 5 months ago by Ambika20
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Gene enrichment analysis
... Hello everyone,, I am working with RNAseq data from a fungi for which we don't have genome annotation. I have a draft genome and list of predicted genes from Augustus. From RNAseq I have list of genes for which I want to do enrichment analysis. I tried to use DAVID for which I tried to get UniProt ...
gene_enrichment david blast2go uniprot written 5 months ago by Ambika20 • updated 5 months ago by Buffo1.1k
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Comment: C: Enrichment analysis of Differentially expressed genes
... Hi, h.mon, I was looking on how can I associate each identified gene with UniProt identifier, but I am lost. Can you suggest a way I can do that. ...
written 5 months ago by Ambika20
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Comment: C: Enrichment analysis of Differentially expressed genes
... Yes you are right I have an assembled draft genome. Right now I am trying to blast my draft genome using Blast2Go and looks like it will take forever. I will try the other idea you said using UniProt. Thank you h.mon for helping. ...
written 5 months ago by Ambika20
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Comment: C: Enrichment analysis of Differentially expressed genes
... No it is not published and not annotated ...
written 5 months ago by Ambika20
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Enrichment analysis of Differentially expressed genes
... Hello everyone, I used STAR-HTseq-DESeq2 pipleline to analyse my RNAseq data and now I have list of differentially expressed genes. I want to do the enrichment analysis of the list of genes obtained from DEseq2. I tried to use DAVID but it didn't work for me since I am working with non-model organ ...
rna-seq enrichment analysis written 5 months ago by Ambika20

Latest awards to Ambika

Popular Question 5 months ago, created a question with more than 1,000 views. For Unable to access jarfile trimmomatic-0.35
Rising Star 10 months ago, created 50 posts within first three months of joining.

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