User: Ambika
Ambika • 20
- Reputation:
- 20
- Status:
- New User
- Location:
- United States/Auburn/Auburn University
- Last seen:
- 5 months, 2 weeks ago
- Joined:
- 1 year, 3 months ago
- Email:
- a***************@gmail.com
Posts by Ambika
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... @Kevin I am just using cDNA to amplify the genes. I did not apply any whole genome amplification ...
written 5 months ago by
Ambika • 20
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... @Kevin Yes I am using ∆∆Ct method to determine the fold change. I collected all my biological samples the same day in order to minimize the variation, and of course the treatments and conditions are same. Even my technical replicates look fine. So, I am having hard time figuring out what is the actu ...
written 5 months ago by
Ambika • 20
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... i already checked the degradation by running gel on my RNA samples before and after DNAse treatment. They look fine. ...
written 5 months ago by
Ambika • 20
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... Hello everyone,
I am trying to validate my RNAseq data by doing qpcr for which I am looking at the fold change of few genes across various timepoints of treatment conditions. I am getting huge amount of variation (in thousand folds ) in my biological replicates. I thought may be it is due to genomi ...
written 5 months ago by
Ambika • 20
• updated
5 months ago by
WouterDeCoster ♦ 35k
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Comment:
C: Gene enrichment analysis
... Thank you I will try that ...
written 7 months ago by
Ambika • 20
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... Hello everyone,,
I am working with RNAseq data from a fungi for which we don't have genome annotation. I have a draft genome and list of predicted genes from Augustus. From RNAseq I have list of genes for which I want to do enrichment analysis. I tried to use DAVID for which I tried to get UniProt ...
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... Hi, h.mon, I was looking on how can I associate each identified gene with UniProt identifier, but I am lost. Can you suggest a way I can do that.
...
written 7 months ago by
Ambika • 20
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... Yes you are right I have an assembled draft genome. Right now I am trying to blast my draft genome using Blast2Go and looks like it will take forever. I will try the other idea you said using UniProt. Thank you h.mon for helping. ...
written 7 months ago by
Ambika • 20
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... No it is not published and not annotated ...
written 7 months ago by
Ambika • 20
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... Hello everyone,
I used STAR-HTseq-DESeq2 pipleline to analyse my RNAseq data and now I have list of differentially expressed genes. I want to do the enrichment analysis of the list of genes obtained from DEseq2. I tried to use DAVID but it didn't work for me since I am working with non-model organ ...
written 7 months ago by
Ambika • 20
Latest awards to Ambika
Popular Question
7 months ago,
created a question with more than 1,000 views.
For Unable to access jarfile trimmomatic-0.35
Rising Star
12 months ago,
created 50 posts within first three months of joining.
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