User: smrutimayipanda

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3 years, 4 months ago
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Posts by smrutimayipanda

<prev • 71 results • page 1 of 8 • next >
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Tool for mycobacterium species
... Hii, I want to annotate mycobacterium houstonense but I didnt get this organism information in dbSNP, NCBI or uniprot. Can you suggest me some tool for mycobacterium species for annotation? or where can I find reference SNPs for annotation? Thanks in advance. ...
next-gen written 7 days ago by smrutimayipanda10
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Comment: C: samtools flagstat interpretation
... I am just asking about the result interpretation. I got your point. ...
written 12 days ago by smrutimayipanda10
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Comment: C: samtools flagstat interpretation
... Hii, I got this result: 20219926 + 0 in total (QC-passed reads + QC-failed reads) 0 + 0 secondary 0 + 0 supplementary 0 + 0 duplicates 91746 + 0 mapped (0.45% : N/A) 20219926 + 0 paired in sequencing 10109963 + 0 read1 10109963 + 0 read2 20252 + 0 properly paired ...
written 12 days ago by smrutimayipanda10 • updated 12 days ago by GenoMax94k
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Comment: C: Trimmomatic adapters for TruSeq Nano DNA kit
... ok thank you so much ...
written 14 days ago by smrutimayipanda10
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Comment: C: Trimmomatic adapters for TruSeq Nano DNA kit
... hii, I got adapter sequences for truseq nano DNA from sequencing providers only but after using these adapters, I am getting 0.05% alignment rate. I used hisat2 for alignment and trimmomatic for adapter trimming. Can you please tell me what's wrong with my process? ...
written 14 days ago by smrutimayipanda10
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Comment: C: Trimmomatic adapters for TruSeq Nano DNA kit
... It is still not working. I am looking for adapter sequences but I am not getting it. From fastqc I got to know that the adapters are from SOLID small RNA adapter. can anyone explain me how to deal with this kind of adapter? Library kit usd is TruSeq nanoDNA kit. ...
written 17 days ago by smrutimayipanda10
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Comment: C: Trimmomatic adapters for TruSeq Nano DNA kit
... i didnt check that. Let me check once ...
written 22 days ago by smrutimayipanda10
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Comment: C: Trimmomatic adapters for TruSeq Nano DNA kit
... I ran adapter trimming by using TruSeq3 but it gave very low alignment rate. Please help me in this ...
written 22 days ago by smrutimayipanda10
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Comment: C: Trimmomatic adapters for TruSeq Nano DNA kit
... yeah I ran fastqc before trimming. Thanks for clarifying. I will definitely check that. ...
written 22 days ago by smrutimayipanda10
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Trimmomatic adapters for TruSeq Nano DNA kit
... Hello, I am working on bacterial data whose library preparation kit used is TruSeq Nano DNA. I am confused which Trimmomatic adapters shall I use for TruSeq Nano DNA kit - TruSeq2 or TruSeq3 or Nextera? Please help me. Thanks in advance. ...
ngs data analysis written 22 days ago by smrutimayipanda10

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