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Posts by valdirbarth
... **Bowtie** seems to be able to do it with this parameter: --nofw/--norc If --nofw is specified, bowtie will not attempt to align against the forward reference strand. If --norc is specified, bowtie will not attempt to align against the reverse-complement reference strand. For paired-end reads usi ...
... Hi all, I am kind of new to bioinformatics and I am trying to understand why I have been getting extremely different results when I trim my sequences to 20 bp vs when I use seed length of 20 on bowtie. (sorry if it is a stupid question, but I need to know) My reads are 75 bp. If I remove adapter s ...
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