User: 1097049297

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10970492970
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Posts by 1097049297

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what do digitisation, offset, and range values mean in fast5 file of Nanopore sequencing
... I recently downloaded an ONT Nanopore sequencing, but I do not know what do this values mean digitisation, offset, and range values could anyone give me some brief answer? Thanks ...
nanopore written 10 months ago by 10970492970
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how to change indel to smallest length in vcf
... I want to annotate VCF with HGMD database information by **sql**, but there is some problem with indel records, such as : >In VCF the variant is `5 13868103 . TA T`, >but in HGMD, it becomes `5 13868104 A .` So for annotation correct, should I change variants like `5 13868103 . TA T` to `5 1 ...
indel annotation next-gen vcf written 18 months ago by 10970492970
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Comment: A: extracting reads from bam file
... As pciifen said, region format is chr:start-stop, like "chr3:1000-2000" ...
written 2.6 years ago by 10970492970
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How to extract reads with a known variant form a bam file
... I want to extract reads with a known variant form a bam file, I have tried like below: ```bash $samtools -q 30 view | grep -i # reference pattern is AA[C]TCG ``` However maybe it would be some problem like if a SNP in the pattern or recurrent pattern in reads So if any one know some better wa ...
bam snp written 2.7 years ago by 10970492970 • updated 2.7 years ago by dariober11k

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Popular Question 18 months ago, created a question with more than 1,000 views. For How to extract reads with a known variant form a bam file

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