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Posts by thaisenayane
... Hello guys, I really need help with one question. In our lab we found a new chromosome break, in our case two genes rearrange within the same chromosome. This was discovered using sanger sequencing and FISH. Since our rearrange is new in literature I need to predict the effect of this change, so I ...
... Hello, I'm trying to perform a differential gene expression analysis using some TCGA data that I reprocessed and my data. However I'm worried about the batch effect. Normally to perform deseq I use this: dds <- DESeqDataSetFromHTSeqCount(sampleTable = d, directory = directory, design= ~ ...
... Hello, I have a paired end library, and I want to fix them after my BWA-MEM alignment on galaxy, however I used "FixMate information" in Picard, this is a good option? or Should I try anything else? maybe "Add or Replace Read goups also in picard"? Thanks ...
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