User: thaisenayane

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Posts by thaisenayane

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Batch effect and DE using TCGA and my data
... Hello, I'm trying to perform a differential gene expression analysis using some TCGA data that I reprocessed and my data. However I'm worried about the batch effect. Normally to perform deseq I use this: dds <- DESeqDataSetFromHTSeqCount(sampleTable = d, directory = directory, design= ~ ...
R rna-seq written 11 days ago by thaisenayane0 • updated 11 days ago by swbarnes26.5k
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Fix Mate Information to PE library
... Hello, I have a paired end library, and I want to fix them after my BWA-MEM alignment on galaxy, however I used "FixMate information" in Picard, this is a good option? or Should I try anything else? maybe "Add or Replace Read goups also in picard"? Thanks ...
next-gen written 21 months ago by thaisenayane0

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