User: anasofiamoreira94

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Posts by anasofiamoreira94

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Comment: C: Insert sequence in nt database
... Can you tell me the header of the sequences that you added? ...
written 4 weeks ago by anasofiamoreira9470
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Comment: C: Insert sequence in nt database
... I'm using blast 2.10.0 version. Thanks for the help... ...
written 5 weeks ago by anasofiamoreira9470
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Comment: C: Insert sequence in nt database
... Ok the I will continue... So I continue to havet NA has a result to taxonomic file... Now I have changed my fasta header files like this: ref|NC_2345|Pleoticus robustu cytochrome c oxidase subunit I (COI) gene, parcial cds; mitochondrial The blast results gives NC_2345 has the ID however in the tax ...
written 5 weeks ago by anasofiamoreira9470
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Comment: C: Create a costum taxonomy file
... i created a new post because the other is extensive... I did the suggested editing but I continue to have NA ...
written 5 weeks ago by anasofiamoreira9470
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Create a costum taxonomy file
... I want to merge some sequences created from my lab with the nt databases. So I did a makeblastdb command and then blastdb_aliastool.However the blast output doesn't give me a taxonomic name, it returns NA. So now, I want to create a costume taxonomy file. Here is an example of my fasta headers: ...
blast written 5 weeks ago by anasofiamoreira9470 • updated 5 weeks ago by genomax85k
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Comment: C: Insert sequence in nt database
... Im creating a new question about this issue ...
written 5 weeks ago by anasofiamoreira9470
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Comment: C: Insert sequence in nt database
... I added that option however I continue to have NA... I created a custom taxonomy file like it was descibred in the page... and then added -taxid_map option to the file name in the makeblastdb option... Could it be the header of the fasta file? ...
written 5 weeks ago by anasofiamoreira9470
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Comment: C: Insert sequence in nt database
... I just managed to add this sequences to my database, but now the blast output gives me NA instead of the specie... Can you help me? ...
written 5 weeks ago by anasofiamoreira9470
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Comment: C: Insert sequence in nt database
... So I don't think I did this right... 1) I did the blastdbcmd and then got 6 files with an extension similar to nt. 2) I did the blastdb_aliastool which generated a .nal file. So I copied this files (step 1 and 2) to my nt database directory. Previously I called nt to my database, by defining a varia ...
written 6 weeks ago by anasofiamoreira9470
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Comment: C: Insert sequence in nt database
... So this is a look of my sequences to introduce in nt database: `>Seq_2 [organism=Pleoticus robustus] COX1 gene, parcial cds,` here I don't put an ID... that's way I'm confused... where to get the ID of this sequence... ...
written 6 weeks ago by anasofiamoreira9470 • updated 6 weeks ago by genomax85k

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