User: lffu_0032

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lffu_003240
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Posts by lffu_0032

<prev • 33 results • page 1 of 4 • next >
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Answer: A: Panel of tumors for CNV detection
... In order to call CNV using catpure daga, you must need the normal or panel or normal data. the tumor data is so complex for its purity and ploidy, so the tumor data is not suit for being the control. ...
written 8 days ago by lffu_003240
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capture-based plasma cnv calling
... Hi, Is there any tools can be used to call CNV in capture-based plasma sequencing data? Thanks a lot. ...
plasma capture-based cnv panel data written 4 weeks ago by lffu_003240 • updated 28 days ago by Kevin Blighe56k
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Comment: A: copy number became inaccurate when downsampling different number fastq reads fro
... I want to known if anyone ever done the downsampling CNV analysis and encountered this problem? ...
written 6 weeks ago by lffu_003240
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Comment: C: copy number became inaccurate when downsampling different number fastq reads fro
... I use seqtk sample command ...
written 6 weeks ago by lffu_003240
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Comment: C: copy number became inaccurate when downsampling different number fastq reads fro
... en, i am not very understand, can you give some detail explan? thank you a lot. ...
written 6 weeks ago by lffu_003240
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Comment: C: copy number became inaccurate when downsampling different number fastq reads fro
... yes, random sampling different number of reads from a full fq data to see which depth is enough to call cnv. ...
written 6 weeks ago by lffu_003240
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Comment: C: copy number became inaccurate when downsampling different number fastq reads fro
... I do not use any thrid-party tools. i just use very simple method: use mean depth normalization to adjust the library size effect, ant calculate the normalized value (depth/mean_depth) for tumor and normal samples, sepeartely. for normal sample, i do not downsampling reads, so the normalized value f ...
written 6 weeks ago by lffu_003240
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Comment: A: copy number became inaccurate when downsampling different number fastq reads fro
... i just use mean depth normalization method to adjust sequencing library size, normalized_depth = target_depth/mean(target_depth), i find for some genes, with smaller downsampling reads, the normalized_depth becames smaller, and for some genes, with smaller downsampling reads, the normalized_depth be ...
written 6 weeks ago by lffu_003240
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Comment: C: copy number increase or decrease WHEN downSample different number fastq reads fr
... we are doing plasma cfDNA sequencing, the sequencing depth is very high (about 10000X-30000X), we want to determine which sequencing depth is enough to call CNV accurately, so we had sequenced some cfDNA samples with ~30000X, and then downsampling to 10000X、15000X、20000X to see which depth is ok to ...
written 6 weeks ago by lffu_003240
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copy number became inaccurate when downsampling different number fastq reads from a whole fastq file
... Hi, everyone: Recent i am doing CNV analysis, i find a strange thing: 1.when using whole data (after removed dup, for tumor and its control), the copy number value is accurate; 2.but when downsample different fq reads from the whole fq for a certain sample (I just downsample the tumor's fq, and ...
copy number not accurate fastq downsample cnv written 6 weeks ago by lffu_003240

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