User: lffu_0032
lffu_0032 • 60
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Posts by lffu_0032
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... the problem is that the naming of Chr in BED and BAM is not same. for example, if the first chr column in your BED is chr1/chr2/chr3..., and the chr column in your bam file is 1/2/3..., then you will meet this "Error in BED line" error. ...
written 4 months ago by
lffu_0032 • 60
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... In order to call CNV using catpure daga, you must need the normal or panel or normal data. the tumor data is so complex for its purity and ploidy, so the tumor data is not suit for being the control. ...
written 10 months ago by
lffu_0032 • 60
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... Hi,
Is there any tools can be used to call CNV in capture-based plasma sequencing data? Thanks a lot. ...
written 10 months ago by
lffu_0032 • 60
• updated
10 months ago by
Kevin Blighe ♦ 69k
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... I want to known if anyone ever done the downsampling CNV analysis and encountered this problem? ...
written 11 months ago by
lffu_0032 • 60
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... I use seqtk sample command ...
written 11 months ago by
lffu_0032 • 60
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... en, i am not very understand, can you give some detail explan? thank you a lot. ...
written 11 months ago by
lffu_0032 • 60
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... yes, random sampling different number of reads from a full fq data to see which depth is enough to call cnv. ...
written 11 months ago by
lffu_0032 • 60
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... I do not use any thrid-party tools. i just use very simple method: use mean depth normalization to adjust the library size effect, ant calculate the normalized value (depth/mean_depth) for tumor and normal samples, sepeartely. for normal sample, i do not downsampling reads, so the normalized value f ...
written 11 months ago by
lffu_0032 • 60
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... i just use mean depth normalization method to adjust sequencing library size, normalized_depth = target_depth/mean(target_depth), i find for some genes, with smaller downsampling reads, the normalized_depth becames smaller, and for some genes, with smaller downsampling reads, the normalized_depth be ...
written 11 months ago by
lffu_0032 • 60
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... we are doing plasma cfDNA sequencing, the sequencing depth is very high (about 10000X-30000X), we want to determine which sequencing depth is enough to call CNV accurately, so we had sequenced some cfDNA samples with ~30000X, and then downsampling to 10000X、15000X、20000X to see which depth is ok to ...
written 11 months ago by
lffu_0032 • 60
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For what is the detailed information of .amb .ann .bwt .pac .sa files generated by the BWA aligner?
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