User: adam.carol

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adam.carol0
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Posts by adam.carol

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Allow mismatches but not gaps in barcode dectection using flexbar
... Hello all, I'm demultiplexing reads from a MiSeq run and I'm having some doubts regarding the barcode detection using Flexbar 3.5. As far as I could understand from the manual, using the parameter '--barcode-error-rate' you can change the threshold for mismatches and gaps, but not one or the other. ...
gap barcode error rate flexbar mismatch written 13 days ago by adam.carol0
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Comment: C: Looking for reputable tool to find low complexity regions in FASTQ or BAM files
... I used masked fasta files to make genome alignments using LASTZ, but I still have a lot of repetitive regions. I wonder if there is any algorithm I can use to remove this repetitive regions in the MAF files that resulted from the alignments. ...
written 9 months ago by adam.carol0
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Filtering repetitive sequences from MAF files
... Hello! Is there any available tool to remove repetitive sequences from genome alignments (MAF format)? The fasta files used in the alignments were downloaded from NCBI and were already masked, but for some reason I still have a lot of repetitive sequences in the resulting MAF files (maybe microsate ...
sequence alignment written 9 months ago by adam.carol0

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