User: blooming.daisy333

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Posts by blooming.daisy333

<prev • 80 results • page 1 of 8 • next >
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determination of alternate splicing events from assembled transcripts
... hi all, I want to determine alternative splicing events for my assembled transcripts (i used strigTie for transcript assembly). my genome is novel for which there is no annotation available in any of the database. i have used ASTALAVASTA software to determine alternate splicing events but this so ...
rna-seq written 5 months ago by blooming.daisy33320
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Comment: C: gene functional analysis using GO and KO numbers
... dear vijay im already upvoting. infact all these answers of nitinnarwade are to the point and realy very helpful. many thanks to him/her. ...
written 5 months ago by blooming.daisy33320
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Comment: C: gene functional analysis using GO and KO numbers
... this is an excellent way of representation. many many thanks for the tool. you ve brought a bbiggner like me out of this juggling stat step by step for which i really appreciate your efforts. but my question was that is there ant key to sub categorize GO terms under 3 basic categories like i gave ...
written 5 months ago by blooming.daisy33320
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Comment: C: gene functional analysis using GO and KO numbers
... Dear nitinnarwade thank you so much for all your kind help and precious time to help me out. as per your suggestion AmiGO GOOSE is giving nice output in the form i need. can you please help me out to resolve one more issue??. In many articles i have found sub categorization of 3 broad categories: ...
written 5 months ago by blooming.daisy33320
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Comment: C: gene functional analysis using GO and KO numbers
... im sorry that im troubling you again but im pretty much confused about the outputs of these programs. let me share one by one: 1) the program like WEGO is something that im looking for but unfortunately its not working and im getting following message while uploading even their demo file : 502 B ...
written 5 months ago by blooming.daisy33320
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Comment: C: gene functional analysis using GO and KO numbers
... sorry for the inconvenience. let me re-explain the question. I have gene ontology (GO IDs) and KEGG pathway (KO annotations both for homology and pathway description). im unable to use these file in GO website to characterize then functionally as this site needs to select pre_listed genome where my ...
written 5 months ago by blooming.daisy33320
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gene functional analysis using GO and KO numbers
... dear all, im interested to find out the functional analysis of my genes (biological process, cellular component and molecular function etc). I do have GO and KO annotations of my gene but could not find out any good software that could give me functional analysis along with graphical representatio ...
rna-seq written 5 months ago by blooming.daisy33320 • updated 3 months ago by Biostar ♦♦ 20
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Comment: C: gene functional analysis
... thanks for the suggestions. PANTHER is very good software but the problem is that it supports only few gene ID formates like uniprot enssemble etc where my geneome annotation is not available. Gene Ontology is powered by PNTHER and I do have GO (Gene ontology) and KO (KEGG) annotations for my gene b ...
written 5 months ago by blooming.daisy33320
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Comment: C: gene functional analysis
... thanks for the kind suggestion. i have tried EggNOG its good but is not giving any visual or graphical representation of the output. further its not summarizing the results in different categories like in biological process and cellular compartments etc. is there any tool that could give me graphic ...
written 5 months ago by blooming.daisy33320
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Comment: C: gene functional analysis
... I mean to annotate your genome in these softwares you need to select the organism in the predefined list while my organism is not listed there hence i cannot use these softwares. im using KEGG database based BlastQUALA where i dont need any reference genome to assess functional annotation of my gene ...
written 5 months ago by blooming.daisy33320

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Rising Star 6 months ago, created 50 posts within first three months of joining.
Supporter 7 months ago, voted at least 25 times.

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