User: apl00028

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apl000280
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Posts by apl00028

<prev • 6 results • page 1 of 1 • next >
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Comment: C: Blast wrong, RNA seq
... These segments are different, they codify for different types of proteins. My NGS sequence is a Cyperus longus plant which have only one segment of this virus. I am going to use these programs, thanks! ...
written 5 weeks ago by apl000280
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Blast wrong, RNA seq
... I am looking for a Virus, which have 3 RNA segments in my NGS data (Cucumber mosaic Virus). I have trimmed adapters to my ngs data and I have convert my fastq files in fasta files which I have done a blastn against a virus database. This blast gave to me a results fairly confuse. It gives to me the ...
ngs rna-seq virus written 5 weeks ago by apl000280 • updated 1 day ago by Biostar ♦♦ 20
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Websites to asses my primers
... I have designed some primers with some degenerated nucleotide which I would like calculate the tm, and the probability of hairpin and self dimer. So far, I have used OligoAnalyzer website but today is out. I would like calculate them with another alternative webiste. Any suggestion? I'd appreciate ...
sequence primer written 8 months ago by apl000280 • updated 7 months ago by Biostar ♦♦ 20
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Comment: C: What program do I must to use to design primer for especific samples?
... Finally I got the consensus sequence all my sequences and I found the primers in NCBI. I did not attempt import them to IGV in BED format because with the tool, find a motive, of IGV is enough to visualize them. Thanks for the answers. ...
written 9 months ago by apl000280
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Comment: C: What program do I must to use to design primer for especific samples?
... yes, I am using this right now, but I would like to visualize them in my mapping view which I got in bowtie2 ...
written 9 months ago by apl000280
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What program do I must to use to design primer for especific samples?
... Hi, I'm trying to design primers for the most conservative region of my sequenced samples of CMV3. I would like design them according with my bowtie2 results (BAM File), which I got with my NGS data. What program do I must to use for my goal? I'd appreciate any piece of information on this issue, t ...
genome alignment primer written 9 months ago by apl000280

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