User: afli

gravatar for afli
afli150
Reputation:
150
Status:
Trusted
Location:
China, Beijing, IGDB
Last seen:
3 days, 8 hours ago
Joined:
6 months, 2 weeks ago
Email:
a***@genetics.ac.cn

Posts by afli

<prev • 111 results • page 1 of 12 • next >
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Comment: C: RNA-seq mapping rate
... Thank you, this sounds reasonable. ...
written 8 weeks ago by afli150
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Comment: C: RNA-seq mapping rate
... I've added the information above. ...
written 8 weeks ago by afli150
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Comment: C: RNA-seq mapping rate
... Thank you for your suggestion. ...
written 8 weeks ago by afli150
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RNA-seq mapping rate
... Hi, I have a basic question about RNA-seq analysis. If reads alignment rate is about 40-50% (from bowties, hisat2, or other alignment tools), would it be appropriate to increase the sequencing depth and get enough aligned reads to do analysis? Or this low alignment rate would cause some bias so we s ...
rna-seq written 8 weeks ago by afli150 • updated 8 weeks ago by Devon Ryan87k
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Comment: C: Too few differentially expressed genes identified by edgeR
... Thank you for your contribution. r1 and r2 mean two biological replicates. ...
written 9 weeks ago by afli150
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Comment: C: Too few differentially expressed genes identified by edgeR
... Thank you for your suggestion, samples _r1 _r2 are two biological replicates. I changed the design but still get no differential genes... library(edgeR) data<-read.table("allh.counts_test.txt",header=T) data<-data[,3:6] Treat <- factor(substring(colnames(data),1,4)) y ...
written 9 weeks ago by afli150
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Comment: C: Too few differentially expressed genes identified by edgeR
... Thank you for your suggestion RamRS, I give the data link and paste the scripts so that everyone can download this data (small size), and run this in edgeR, totally need about 2 minutes. And I edit my post with more detail for those who do not have much time to run and check my data. Hope this is OK ...
written 9 weeks ago by afli150
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Too few differentially expressed genes identified by edgeR
... Dear all, I'm new in using edgeR. I get no diffrentially expressed genes and I don't know why, could anyone give me some advice? Thank you very much! I pasted the data link and my scripts as follows. data link: https://de.cyverse.org/dl/d/5F987820-8F84-4772-8561-416202A11580/allh.counts_test.txt ...
edger written 9 weeks ago by afli150
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Comment: C: Software to visualize ChIP-seq peak file
... Thank you for your contribution! ...
written 9 weeks ago by afli150
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Comment: C: Software to visualize ChIP-seq peak file
... Yeah, this is a very good idea! I checked this and get the expected pdf file. Thank you! ...
written 9 weeks ago by afli150

Latest awards to afli

Centurion 11 weeks ago, created 100 posts.
Scholar 11 weeks ago, created an answer that has been accepted. For C: DEseq2 logfold interpretation
Scholar 12 weeks ago, created an answer that has been accepted. For C: DEseq2 logfold interpretation
Scholar 3 months ago, created an answer that has been accepted. For A: Compare expression of one group to the mean of others using DESeq2
Teacher 3 months ago, created an answer with at least 3 up-votes. For C: DEseq2 logfold interpretation
Scholar 3 months ago, created an answer that has been accepted. For A: Compare expression of one group to the mean of others using DESeq2
Rising Star 4 months ago, created 50 posts within first three months of joining.

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