User: greyman

gravatar for greyman
greyman30
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1 month ago
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2 months, 2 weeks ago
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Posts by greyman

<prev • 14 results • page 1 of 2 • next >
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Comment: C: Can we find SNP from fasta sequences ?
... As i wanted to extract the sequence region with SNP from exome data, is there anyway to do it from fastq? Last time when i use fasta i extract the gene sequence from whole genome data that has been split into gene data ...
written 9 weeks ago by greyman30
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Can we find SNP from fasta sequences ?
... I found the SNP from bam file and vcf workflow however I would like to try using FASTA for this process. Can we get Fasta format works? ...
fasta snp written 9 weeks ago by greyman30
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Comment: C: How to work with 2.6 TB genome data which is deposited in the server?
... Thanks genomax!! i would like to work on all data in once but it seems like i have to separate them into batch ...start searching about dual socket workstation like HP Z820 Tower Workstation 16-Core E5-2670 (16 cores, hard disk storage15TB, RAM32 GB).... and looks like i ll have to add more RAM to ...
written 9 weeks ago by greyman30
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Comment: C: How to work with 2.6 TB genome data which is deposited in the server?
... They dont own a HPC but they are interested to do modification to a Lenovo desktop that i m currently using. The 7k dollar would be invested in the modification, however, i ll request for more fund if buying a new desktop is necessary. They are expecting to see some fruitful output from the 2.6TB be ...
written 9 weeks ago by greyman30
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Comment: C: How to work with 2.6 TB genome data which is deposited in the server?
... Hi, thank you so much for your reply and i was asked to submit a grant proposal regarding CPU, RAM and HDD and probably a desktop in two days time. As the desktop gonna be placed at my personal workbench, with budget 7000 dollar, would it be possible to hear some advice from you in getting these har ...
written 9 weeks ago by greyman30
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Comment: C: How to get quality output for exome assembly ?
... thank you so much! I have no much information about adaptor sequence, only know that it generated by Hiseq sequencing. Would you think Trimmomatic using ILLUMINACLIP: Truseq gonna work? Would u recommend to try on BBDUK? Will input the BAM into genome viewer. There are @SQ in my BAM , how would y ...
written 10 weeks ago by greyman30
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Comment: C: How to work with 2.6 TB genome data which is deposited in the server?
... thank you, still learning... ...
written 10 weeks ago by greyman30
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Comment: C: How to get quality output for exome assembly ?
... thank you, just change it ...
written 10 weeks ago by greyman30
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How to get quality output for exome assembly ?
... Hi all I am very new in exome seqeucing for SNP variants calling. I got my overall alignment rate of 97.02%. Can Trimmomatic do exome trimming for Hiseq PE? i didnt have information about the sequencing cycle.... Also, from fastqc reports that my per base sequence quality is fine but the "per base ...
exome samtools sequencing written 10 weeks ago by greyman30
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Comment: C: How to work with 2.6 TB genome data which is deposited in the server?
... Thank you, it is population disease data ...
written 10 weeks ago by greyman30

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