User: joelslade

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joelslade10
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Posts by joelslade

<prev • 5 results • page 1 of 1 • next >
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Comment: C: Program for Amplicon (MHC) Analysis?
... I see that your files were SOSP MHC -- that is what I did for my PhD. Neat. ...
written 6 weeks ago by joelslade10
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Answer: A: Program for Amplicon (MHC) Analysis?
... Unfortunately, these aren't SNPs but entire sequences. I know there are some HLA-specific programs, but many of them are only based for human MHC. ...
written 6 weeks ago by joelslade10
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Program for Amplicon (MHC) Analysis?
... Hi there, I have been trying out different programs to analyze amplicons sequenced by an Illumina MiSeq. Originally I gave AmpliSAS a try, and the program seems to eliminate too many MHC alleles from each individual, and only allows uploads of 500mb files. I thought mothur may be a good program t ...
gene mhc written 6 weeks ago by joelslade10
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Comment: A: Ilumina V2 vs V3 for amplicon sequencing
... Thanks so much for your response. I must be confused by the terminology. I just assumed that V3 meant 2 x 300 bp, but does it just mean you can only use V3 to do 2 x 300 bp, while V2 is reserved for the lower read lengths? I assume V2 2 x 250 bp is the most robust? ...
written 5 months ago by joelslade10
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Ilumina V2 vs V3 for amplicon sequencing
... Hi there, I am having a dilemma -- I am using an Illumina MiSeq to sequence MHC in songbirds (which can have many alleles per individual). I was able to create a primer to get sequences that range from 370-420 bp (due to indels and differences between subspecies). This means some sequences will not ...
illumina amplicon next-gen sequencing written 5 months ago by joelslade10

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