User: mbk0asis

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mbk0asis510
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510
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Korea, Republic Of
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8 hours ago
Joined:
7 years, 8 months ago
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Posts by mbk0asis

<prev • 137 results • page 1 of 14 • next >
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Comment: C: Tool to design Q-PCR primers with exon skipping?
... Primer3 has an option "Excluded Regions" to skip the regions you want. You can specify the length and positions of the Excluded regions. Read following instruction! > E.g. 401,7 68,3 forbids selection of primers in the 7 bases starting at 401 and the 3 bases at 68. Or mark the source sequence ...
written 13 hours ago by mbk0asis510
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Comment: C: How to plot 2 different symbols in a 3D PCA plot generated by rgl-plot3D
... I think 'plot3D' and 'plot3d' are different. Former one is a package, while the later is a function. ...
written 1 day ago by mbk0asis510
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Comment: C: Looking for the soft that generated this figure
... I'm not sure there is a software for such plots, but I think geom_point (ggplot2) can do the job if you can obtain the data. ...
written 1 day ago by mbk0asis510
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Comment: C: Normalizing NGS samples expected to have different total reads
... I found the a sentence, "It has been proposed that quantile normalization can correct for varying DNA enrichment efficiencies." in the MEDIPS vignette. However, this seems different from my case. I think the quantile normalization would be useful when there is difference in sequencing depth over t ...
written 2 days ago by mbk0asis510
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Comment: C: Normalizing NGS samples expected to have different total reads
... Thank you for the advice! I will check that out. ...
written 9 days ago by mbk0asis510
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Normalizing NGS samples expected to have different total reads
... Hello! I have MBD-seq datasets of Dnmt1 Knock-Out and control cells. As expected, Dnmt1 KO covered very few genomic regions compared to control sample since no Dnmt1 presented in the KO. The problem is that the reads in the KO sample were concentrated in the "few genomic region" made the intensit ...
normalization ngs written 9 days ago by mbk0asis510
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Comment: C: fGSEA result to csv
... Thnanks! It worked!! ...
written 6 weeks ago by mbk0asis510
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6 follow
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fGSEA result to csv
... Hi, all! I've obtained the enriched GO BP terms using fGSEA in R as shown below. 1: GO_POSITIVE_REGULATION_OF_VIRAL_TRANSCRIPTION 0.020378457 0.08797654 0.4748196 1.5113374 13 41 TFAP4,NELFCD,POLR2H,POLR2D,NELFA,DHX9,... 2: GO_CARDIAC_CHA ...
fgsea rna-seq gsea written 7 weeks ago by mbk0asis510 • updated 6 weeks ago by alserg320
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Comment: C: WGCNA package in R: dendogram plot error
... How can I extract gene lists from blocks and dendros? ...
written 7 weeks ago by mbk0asis510
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WGCNA blockwiseConsensusModules : number of colors and objects in dendrogram don't match
... Hi. I'm running WGCNA to find consensus modules between two experiments. I'm stuck at 'plotDendroAndColor' step due to the mismatch of the number of 'consTree' and 'moduleColors' as shown below. As a result, the plot showed only the dendrogram but no colored modules. > length(moduleColor ...
wgcna blockwiseconsensusmodules written 7 weeks ago by mbk0asis510

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Voter 9 days ago, voted more than 100 times.
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Epic Question 11 months ago, created a question with more than 10,000 views. For Finding a sequence in a fasta file
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