User: baldissera152
baldissera152 • 10
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Posts by baldissera152
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C: DESeq2 design and Batch effects
... Thank you for your reply.
You are right, it's very hard to analyze data when the design is very restrict. The groups I have defined as "acute" and "later" were based on the original paper's findings. My PCA just confirmed a grouping tendency.
Regarding my idea of splitting a group based on the PCA ...
written 5 days ago by
baldissera152 • 10
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... Hi, guys.
I am analyzing some RNA-Seq data from animals in different time points after a trauma in the spinal cord. The animal’s tissue have been pooled according to the time-points, therefore I do not have biological neither technical replicates.
In order to analyze this data, I have attributed a ...
written 9 days ago by
baldissera152 • 10
• updated
9 days ago by
ATpoint ♦ 26k
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... Hi, guys
I intend to to integrate two single-cell RNA-Seq datasets and then correct their expression values for batch effect using Scran mnnCorrect function. However, after mnn correction, the output is a matrix which includes logtransformed and negative values. Since softwares usually require raw ...
written 13 months ago by
baldissera152 • 10
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