User: RS

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RS0
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Posts by RS

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Comment: C: what is difference between strand specific in rnaseq analysis from htseq-count
... Hello Devon, During counting the reads I have done one mistake that I counted with unstranded option, however my samples were from reverse stranded. The percentage of mapping was 80% for unstranded and 98% for reverse. But for DEGs I can see there is very less difference. Is it due to the other stra ...
written 5 months ago by RS0
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what is the best method (normalization) to compare RNA-Seq samples from different labs
... Hello, I have more than 100 RNA-Seq samples (files) from different labs (i.e, different papers) for plant tissues. I want to compare them (for DEGs, PCA, tSNE, absolute expression, etc.). But for this, the data should be normalized. Data are from different labs, thus I want to take special care for ...
normalization compare rna-seq tmm written 8 months ago by RS0 • updated 8 months ago by Dattatray Mongad330
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Do downloaded fastq files from NCBI (SRA) needs preprocessing before used in bowtie2 and featureCounts
... I am new to RNA-Seq data analysis. Sorry for very basic question :) I was given a task to generate read counts for a published dataset. I have followed the below pipeline and generated read counts. But in this pipeline there is no preprocessing of fastq files like removal of ribosome sequences. are ...
rna-seq sequencing written 10 months ago by RS0 • updated 10 months ago by finswimmer12k

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