User: rprog008

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rprog0080
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Posts by rprog008

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Comment: C: identify individuals for which the levels of the malignant isoform (m) significa
... I have solved the problem :) ...
written 5 days ago by rprog0080
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identify individuals for which the levels of the malignant isoform (m) significantly change independently
... I am performing similar work as stated in https://github.com/mpg-age-bioinformatics/exercise but with neurological disorder in mouse model. I have generated all file stated in the website but I am not understanding how to identify individuals for which the levels of the malignant isoform (m) signifi ...
R rna-seq written 7 days ago by rprog0080
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Comment: A: Shall I take vigna angularis or vigna radiata as a reference for vigna munga
... Thanks @natasha.sernova and @ genomax I found one literature, https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0147568, where they have reported Vigna munga is closely related with Vigna radiata ( figure 3 and 4) :) Is it ok, if I take vigna radiata for annotating vigna mungo seque ...
written 25 days ago by rprog0080
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Comment: C: Shall I take vigna angularis or vigna radiata as a reference for vigna munga
... [edited] Yes three genomes of three species namely V. angularis, V. radiata and V. unguiculata are available :) Thanks I will do try to build phylogenetic tree and proceed further now :) ...
written 25 days ago by rprog0080
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Comment: C: Shall I take vigna angularis or vigna radiata as a reference for vigna munga
... Thank you for your reply :) Yes, you are right. At first I have assembled my data with trinity. Now I want to annotate the assembled transcript. ...
written 25 days ago by rprog0080
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Comment: A: Can I perform de novo analysis on RNA seq file of Mus musculus?
... Thanks @kristoffer and @JC for your reply. @Kristoffer.. This workflow look great. I will try. One question i have .. will it be ok, if i assemble transcript file using Trinity and annotate those transcript with mouse genome. ...
written 25 days ago by rprog0080
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Shall I take vigna angularis or vigna radiata as a reference for vigna munga
... Dear member, I want to annotate transcript sequence (FASTA file) of Vigna Mungo obtained from Trinity. In NCBI there is reference genome of Vigna radiata and Vigna angularis. Can anyone please suggests which one shall I take and why. Thanks in advance ...
annotation rna-seq written 25 days ago by rprog0080
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Can I perform de novo analysis on RNA seq file of Mus musculus?
... Dear member, I got some interesting SRA file of Mus musculus related with my work in SRA database. Original authors have utilised Trapnel protocol for their analysis and found some interesting result but I found some lacuna, like no analysis of long non codind RNA analysis. ...
de novo rna-seq differential expression written 25 days ago by rprog0080
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Did both mitochondrial DNA and the Y chromosome coalesce within the last several hundred thousand years ?
... Did mitochondrial DNA and the Y chromosome coalesce within the last several hundred thousand years. I am not understanding how mt and Y chromosome have merged in human. Can anyone please explain me this line ? I am following this paper. https://www.pnas.org/content/95/4/1961 ...
demography human population genetics written 7 weeks ago by rprog0080
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Answer: A: MD into bioinformatics
... if you want you can do MS in bioinformatics. But remember getting a degree does not make you bioinformatician. Your active research in bioinformatic make you bioinformatician. If you are interested in Bioinformatics and as already you have completed your MD and PhD, try to figure out research topic ...
written 7 weeks ago by rprog0080

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