User: rainwashautumn

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Posts by rainwashautumn

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Comment: C: SPLiT-Seq Demultiplexing a bash tool for extraction of barcoded single cells
... Thanks a lot for your meticulously reply. Now I have noticed that the full dataset which is used for benchmarking contains only 131 nuclei (according to the paper )while your script generated 14,423 .fastq files (cells) as output. I am new to bioinformatics and hope my question do not bother you ...
written 28 days ago by rainwashautumn0
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Comment: A: SPLiT-Seq Demultiplexing a bash tool for extraction of barcoded single cells
... Hello, firstly I would like to appreciate for your work. But there is some doubts about the Round*_barcodes_new.txt files. I am curious about why round1 barcode is 13 bits and 2 is 13 bits, 3 is 14or 15 bits. We know that the round1 unique barcode is 8bits, yet you add 5 redundant bits which is tot ...
written 6 weeks ago by rainwashautumn0

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