User: umeshtanwar2

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umeshtanwar210
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Posts by umeshtanwar2

<prev • 6 results • page 1 of 1 • next >
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Comment: C: RNA-Seq Differential gene expression
... Thank you seidel for you kind suggestion. I will go through the edgeR userguide. ...
written 5 days ago by umeshtanwar210
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RNA-Seq Differential gene expression
... Hi, I am working on plant Arabidopsis. I have the samples for 4 plant types (WT, KO1, KO2 and OE1) in three replicates, Mock treated and after stress treatment. So in total 24 samples (4 Mock and 4 Treated, 3 replicates of each). I would like to analyse the differential gene expression in each sampl ...
rna-seq written 5 days ago by umeshtanwar210
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Comment: C: Transcriptome analysis for differential gene expression
... Thank you Nicolas Rosewick for your kind help. ...
written 6 days ago by umeshtanwar210
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Transcriptome analysis for differential gene expression
... Hi. I am new for performing RNA-Seq. I have got the sequencing raw reads (PE) for my samples from Arabidopsis thaliana plants. I have analysed these reads using FASTQC. Now I have to do the alignment and mapping of these reads with the refrence genome. In this regard my first question is, should ...
rna-seq written 6 days ago by umeshtanwar210 • updated 6 days ago by Nicolas Rosewick7.2k
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Comment: C: STAR for Genome Index and Alignment
... Thank you so much genomax. It started working. ...
written 7 days ago by umeshtanwar210
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STAR for Genome Index and Alignment
... Hi. I am new for using STAR. I am generating the genome index for Arabidopsis thaliana (ftp://ftp.ensemblgenomes.org/pub/release-41/plants/fasta/arabidopsis_thaliana/dna/). I downloaded the FASTA files for each chromosome and the GTF file. My script was: STAR --runThreadN 1 --runMode genomeGen ...
rna-seq written 7 days ago by umeshtanwar210 • updated 7 days ago by genomax60k

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