User: SDin

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SDin0
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Posts by SDin

<prev • 11 results • page 1 of 2 • next >
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Comment: C: How can MMLV enzyme add cytosines to the 3' of cDNA
... Sorry, I am not familiar with the rule of this site. I apologize. And thanks for your advice. ...
written 7 months ago by SDin0
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(Closed) How can MMLV enzyme add cytosines to the 3' of cDNA
... Hi there, I am working on the mechanism of *SMART* , a kind of reverse transcription strategy. However, I am confused about the terminal transferase activity of MMLV enzyme. It have been reported since 1998, but I cannot find any resource to figure out HOW can it add Cs on the 3' of cDNA. Can anyo ...
next-gen rna-seq sequencing written 7 months ago by SDin0
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Comment: C: Pysam.view report 'pysam.utils.SamtoolsError: "samtools returned with error 1: s
... I decide to use command line mode of samtools at last, thanks for your kind help. ...
written 7 months ago by SDin0
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Comment: C: Pysam.view report 'pysam.utils.SamtoolsError: "samtools returned with error 1: s
... Thanks for your kind help, it can run now. But when I run the code, there are some messy code on the screen: @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@\x00\xdbx:Z\xe0\x9d\x00\x00\x1f\x8b\x08\x04\x00\x00\x00\x00\x00\xff\x06\x00BC\x02\x00\x1b\ ...
written 8 months ago by SDin0
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Comment: C: Pysam.view report 'pysam.utils.SamtoolsError: "samtools returned with error 1: s
... Hmmm, no, the 'samtools view' works. So it is quite weird.. ...
written 8 months ago by SDin0
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Comment: C: Pysam.view report 'pysam.utils.SamtoolsError: "samtools returned with error 1: s
... It is already in the command line.. ...
written 8 months ago by SDin0
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Pysam.view report 'pysam.utils.SamtoolsError: "samtools returned with error 1: stdout=, stderr=samtools view: invalid option -- ' ''
... Hi there, I am a beginner in this area. I am trying to use pysam for extract some reads. My code is pysam.view("-u -f 8 -F 260","/usr/local/virussite/input.bam","-o","output2.bam") and pysam.view("-u -f 8 -F 260","/usr/local/virussite/input.bam > output2.bam") both will report ...
samtools pysam written 8 months ago by SDin0 • updated 8 months ago by ATpoint25k
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Questions from beginner: Barcode of Single cell RNA-seq
... Hi there, I am a freshman in this area. When I reading the review of it, I am a little bit confused by the barcode of it. In the review, it said barcode is added by PCR. So it is on the end of the sequence. My question is: When we doing the NGS, the sequences will go through 'fragmentation' step ...
next-gen rna-seq sequencing written 8 months ago by SDin0
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Comment: C: extracting the soft clipped seq only from a sam file
... Hi, IrK, sorry for bothering you. Have you solved this problem? It will be very appreciated that you can give some advice. ...
written 8 months ago by SDin0
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Comment: C: Samtools: Question about filtering BAM file using flag
... I got it. Many thanks for your kind help! ...
written 8 months ago by SDin0

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