Moderator: Ryan Dale
Ryan Dale ♦ 4.8k
- Reputation:
- 4,850
- Status:
- Trusted
- Location:
- Bethesda, MD
- Website:
- https://github.com/daler
- Twitter:
- @daler_t
- Scholar ID:
- Google Scholar Page
- Last seen:
- 1 year, 8 months ago
- Joined:
- 9 years, 4 months ago
- Email:
- d*****@niddk.nih.gov
A benthic ecologist by training, but now a bioinformatician at NIH's National Institute of Diabetes and Digestive and Kidney Diseases, Laboratory of Cellular and Developmental Biology.
Currently studying the relationship of chromatin conformation and gene regulation.
Please note: any opinions expressed here are my own and not those of my employer.
Posts by Ryan Dale
<prev
• 215 results •
page 1 of 22 •
next >
0
votes
2
answers
2.6k
views
2
answers
Comment:
C: ChromHMM Output Descriptions
... The label of each state is subjective. Coming up with good labels requires looking carefully at the enrichments (from running OverlapEnrichment) and emissions heatmaps to decide what you want to name them. ...
written 2.2 years ago by
Ryan Dale ♦ 4.8k
1
vote
3
answers
1.3k
views
3
answers
... Sounds like you're running on a headless server with no X11 windows. In that case add these two lines to the very beginning:
import matplotilb
matplotlib.use('agg')
see http://stackoverflow.com/questions/2801882/generating-a-png-with-matplotlib-when-display-is-undefined for more details an ...
written 2.6 years ago by
Ryan Dale ♦ 4.8k
2
votes
3
answers
1.3k
views
3
answers
... If you have enough RAM and are OK with Python:
import pybedtools
from matplotlib import pyplot as plt
sizes = [len(i) for i in pybedtools.BedTool('my.bed')]
plt.hist(sizes)
plt.savefig('hist.png')
plt.show()
...
written 2.6 years ago by
Ryan Dale ♦ 4.8k
2
votes
2
answers
1.1k
views
2
answers
... The `gffutils` python package can help with this: https://daler.github.io/gffutils
It will infer the gene and transcript extents; doing so is the default behavior for GTF files. The following will import the GTF into a sqlite3 database (which you can use for other things if needed) and then output ...
written 2.6 years ago by
Ryan Dale ♦ 4.8k
0
votes
1
answer
1.6k
views
1
answers
... As you mention, ChromHMM expects read counts. Even if you transform your data somehow to make it work with ChromHMM, how will you interpret the results? I would expect the HMM to see the imputed data as a combination of the other vars anyway, making it redundant. I guess I'm not sure there's an adva ...
written 2.6 years ago by
Ryan Dale ♦ 4.8k
1
vote
1
answer
2.1k
views
1
answers
... FYI, versions 0.4.4 thru 0.7.0 are available for Linux & OSX via [bioconda][1]
[1]: https://bioconda.github.io ...
written 2.7 years ago by
Ryan Dale ♦ 4.8k
1
vote
0
answers
1.2k
views
0
answers
... We have an opening for a post-baccalaureate bioinformatics trainee in the Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases at the National Institutes of Health in Bethesda, MD. This is a one year appointment, starting as soon as May 2 ...
4
votes
1
answer
3.4k
views
1
answers
Answer:
A: ChIP-seq vs. RNA-seq
... Map them both to a reference; the RNA-seq library will be more likely to have splice junctions (look for "N" in the BAM cigar string) and will be sparse in intergenic regions, while the ChIP-seq will have more even coverage, less dynamic range, and will probably have some low-level signal in most pl ...
written 2.9 years ago by
Ryan Dale ♦ 4.8k
1
vote
5
answers
3.9k
views
5
answers
... Fidel, this is amazing. A much-needed and much-appreciated initiative. ...
written 2.9 years ago by
Ryan Dale ♦ 4.8k
0
votes
2
answers
3.6k
views
2
answers
... Are you using a Clontech kit by any chance? ...
written 3.1 years ago by
Ryan Dale ♦ 4.8k
Latest awards to Ryan Dale
Appreciated
2.1 years ago,
created a post with more than 5 votes.
For A: How To Plot/Visualise Tfbs Positions Relative To Tss For Thousands Of Genes?
Teacher
2.5 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Scholar
2.6 years ago,
created an answer that has been accepted.
For A: Making gene annotation from a GTF file
Scholar
2.6 years ago,
created an answer that has been accepted.
For A: Creatig TrackHubs from BigWigs
Popular Question
2.7 years ago,
created a question with more than 1,000 views.
For Bioinformatics trainee position at NIH
Teacher
2.8 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Teacher
2.9 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Scholar
3.2 years ago,
created an answer that has been accepted.
For A: Making gene annotation from a GTF file
Teacher
3.2 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Teacher
3.3 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Scholar
3.3 years ago,
created an answer that has been accepted.
For A: Making gene annotation from a GTF file
Teacher
3.3 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Teacher
3.3 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Scholar
3.3 years ago,
created an answer that has been accepted.
For A: Making gene annotation from a GTF file
Good Answer
3.4 years ago,
created an answer that was upvoted at least 5 times.
For A: Samtools View - What'S The Purpose Of The -T Option?
Good Answer
3.4 years ago,
created an answer that was upvoted at least 5 times.
For A: Samtools View - What'S The Purpose Of The -T Option?
Scholar
3.4 years ago,
created an answer that has been accepted.
For A: Making gene annotation from a GTF file
Teacher
3.4 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Appreciated
3.5 years ago,
created a post with more than 5 votes.
For A: How To Plot/Visualise Tfbs Positions Relative To Tss For Thousands Of Genes?
Teacher
3.5 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Scholar
3.5 years ago,
created an answer that has been accepted.
For A: Making gene annotation from a GTF file
Appreciated
3.5 years ago,
created a post with more than 5 votes.
For A: How To Plot/Visualise Tfbs Positions Relative To Tss For Thousands Of Genes?
Teacher
3.5 years ago,
created an answer with at least 3 up-votes.
For A: Visualizing Genomic Interactions
Good Answer
3.5 years ago,
created an answer that was upvoted at least 5 times.
For A: Samtools View - What'S The Purpose Of The -T Option?
Scholar
3.5 years ago,
created an answer that has been accepted.
For A: Making gene annotation from a GTF file
Use of this site constitutes acceptance of our User
Agreement
and Privacy
Policy.
Powered by Biostar
version 2.3.0
Traffic: 1852 users visited in the last hour