User: suvratha

gravatar for suvratha
suvratha40
Reputation:
40
Status:
New User
Location:
Ulm
Twitter:
SuvrathaPrasad
Last seen:
2 days, 13 hours ago
Joined:
1 year, 2 months ago
Email:
s*******@gmail.com

Posts by suvratha

<prev • 31 results • page 1 of 4 • next >
0
votes
1
answer
131
views
1
answers
Comment: C: Gradient bar plot in ggplot2
... is there a function to use the gradient colours as different shades of grey with a white background? ...
written 25 days ago by suvratha40
0
votes
1
answer
131
views
1
answers
Comment: C: Gradient bar plot in ggplot2
... Thank you very much! ...
written 26 days ago by suvratha40
3
votes
1
answer
131
views
1
answer
Gradient bar plot in ggplot2
... I've 3 variables, for a set of genes - the log2FC, the log2(basemeanB) and the difference in expression of a gene in fraction of cells. below are my vectors - df <- data.frame(Genes=c('ADCYAP1R’, ‘GRM1’, ‘GRM3’, ‘HTR1F’, ‘HTR2A’, ‘MCHR2’, ‘RXFP1’), Log2FC=c(-2.299, 0.52, 2.439, 1.783, 2.459 ...
R ggplot2 rna-seq written 26 days ago by suvratha40 • updated 26 days ago by venu6.6k
0
votes
1
answer
778
views
1
answers
Comment: C: Could you please tell me how should I analyze the difference between KO and WT m
... can Cell Ranger do differential expression in its pipeline or do you have to use Seurat for doing differential expression analysis? ...
written 5 weeks ago by suvratha40
0
votes
1
answer
212
views
1
answers
Comment: C: Differential expression testing in Seurat
... The only way to know that would be by doing what you suggested in your previous comments? ...
written 6 weeks ago by suvratha40
0
votes
1
answer
212
views
1
answers
Comment: C: Differential expression testing in Seurat
... I see, thank you for the explanation. My question still remains - which one do i pick for differential expression testing? Wilcoxon test or DESeq2? ...
written 6 weeks ago by suvratha40
0
votes
1
answer
212
views
1
answers
Comment: C: Differential expression testing in Seurat
... so which results should i report? the one from Wilcoxon test or from DESeq2? Also the number of differentially expressed genes I get from DESeq2 is way more than the number of genes I get from the Wilcoxon test, so I don't know which ones to trust. i.e. - the genes I'm looking into is gets detected ...
written 6 weeks ago by suvratha40
0
votes
1
answer
212
views
1
answers
Comment: C: Differential expression testing in Seurat
... so then what's the point of the `ScaleData()` function? ...
written 6 weeks ago by suvratha40
0
votes
1
answer
212
views
1
answer
Differential expression testing in Seurat
... As I've understood Seurat properly, in the initial steps, it performs scaling and centering of the data so that your data resembles a normal distribution using the `ScaleData()` function. If this is the case, then why does the default test for differential expression have the Wilcoxon test when it i ...
R rna-seq seurat written 6 weeks ago by suvratha40 • updated 6 weeks ago by ATpoint34k
0
votes
1
answer
189
views
1
answers
Comment: C: DESeq2 Error in data.frame(row.names = colnames(countdata), condition) : row nam
... thank you, this fixed it! ...
written 7 weeks ago by suvratha40

Latest awards to suvratha

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 796 users visited in the last hour