User: edwardbeaty

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Posts by edwardbeaty

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Downloading Ncbi Query Results Using Aspera?
... Hi, all. I have a very large result set (150k results) from NCBI Entrez that I want to download as genbank files. I just found the Aspera plugin for file transfer...does anyone know if it's possible to download very large query results using this tool? Thanks. ...
ncbi aspera written 5.6 years ago by edwardbeaty0 • updated 4.2 years ago by Casey Bergman17k
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Comment: C: Retrieving Genbank Xml Through Efetchseq (Or Another Entrez Web Service?)
... Thanks, I might try the REST service. I didn't see a "retmode" parameter either; also there wasn't an obvious way to pull the results from the eFetchResult object that gets returned. ...
written 5.6 years ago by edwardbeaty0
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Retrieving Genbank Xml Through Efetchseq (Or Another Entrez Web Service?)
... Hello! I have a question about using the Entrez Utility Web Service to retrieve records in genbank XML format. I’m using C# to access the eFetchSeq service at http://eutils.ncbi.nlm.nih.gov/soap/v2.0/efetch_seq.wsdl like so: eFetchSeq.eUtilsServiceSoapClient serv = new eFetchSeq.eUtilsServiceS ...
entrez written 5.6 years ago by edwardbeaty0 • updated 5.6 years ago by Pierre Lindenbaum112k
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Tools For Calculating Accurate Primer-Target Tm'S (With Mismatches)?
... Hi! I just found this site from reddit, and thought I'd ask this question here as well. I have a large number of DNA primer-target pairs for which I need to calculate the melting temperature. The trick is that some of the pairs will have mismatches, and I want the numbers to as closely reflect phys ...
primer written 6.3 years ago by edwardbeaty0 • updated 2.1 years ago by Biostar ♦♦ 20

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