User: Biological information research group of Harbin medical university daqing campus

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Posts by Biological information research group of Harbin medical university daqing campus

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Comment: A: Integrating scRNA-seq datasets with different numbers of biological replicates
... If you want to integrate several different scrna-seq data sets, you can use Seurat3. ...
written 11 weeks ago by Biological information research group of Harbin medical university daqing campus10 • updated 11 weeks ago by ATpoint29k
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Answer: A: Identification of Human Genes ?
... You can go to GENCODE and download an annotated file of a human gene and intersect his gene with the gene in the annotated file using the R function intersect () or go to ensemble Biomart and convert the ID here is the website of [Biomart][1] [1]: https://www.oebiotech.com/Article/ensemblbio.htm ...
written 12 weeks ago by Biological information research group of Harbin medical university daqing campus10 • updated 11 weeks ago by ATpoint29k
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Comment: C: get genomic information for probe sequence
... I have another idea that I hope can help you.Sequence alignment was made with fa file and probe sequence of reference genome sequence and mrna reference sequence respectively, and then python code was written to extract location information from alignment result XML or other format files.The probe s ...
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Comment: C: Tools for scRNAseq
... Seurat,Linnorm,Dimm-SC,It can all be done, and I'm glad it helps ...
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Comment: C: huge difference of expression value of different probes for the same gene. Why?
... This can answer from the perspective of alternative splicing, for a specific gene, follow specific probe design in the gene, and because of the different regions of the same gene the use efficiency of alternative splicing sites in the same cell condition is different, will eventually lead to differe ...
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Answer: A: Epistatic effect for two alleles
... Why is there a lot of functionality that's not working right now with my biostar? How about bookstore and thumb up? thank you ...
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Comment: C: Tool to separate human and mouse rna seq reads
... I have a personal thought, if it's not correct then I'm sorry. If you know which is the mouse and which is the human in the data, you can write shell split directly. If you don't know, you can compare it first and then split it after comparing it twice. ...

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