User: Wang

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Wang10
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8 months, 2 weeks ago
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1 year, 7 months ago
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c*******@163.com

Posts by Wang

<prev • 11 results • page 1 of 2 • next >
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Comment: C: Aspera ascp command line utility inside prefetch call to facilitate SRA data dow
... Thank you for sharing this information. Bad news.It's too slow via https. Any suggestion to accelerate the download? ...
written 8 months ago by Wang10
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Comment: C: Why ranges of narrowPeak file and that of bed file are not identical
... OK,thank you.May be I missed some useful information.I'll read these again. ...
written 18 months ago by Wang10
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Comment: C: Why ranges of narrowPeak file and that of bed file are not identical
... Sorry! I'm newbie here. Maybe I should edit the original post and append these informations. ...
written 18 months ago by Wang10
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Comment: C: Why ranges of narrowPeak file and that of bed file are not identical
... Thank you,I really appreciate it! Is there any detailed materials or article I can read? I failed to find these information in the official README.md ...
written 18 months ago by Wang10
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Comment: A: Why ranges of narrowPeak file and that of bed file are not identical
... I run macs2 this way macs2 callpeak -t ff.bam -c input.bam -n ff -f BAM -g mm `ff.bam` and `input.bam` was sorted by samtools samtools sort -@ 32 1.bam >ff.bam samtools sort -@ 32 2.bam >input.bam ...
written 18 months ago by Wang10 • updated 18 months ago by _r_am31k
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Comment: C: Why ranges of narrowPeak file and that of bed file are not identical
... ok,I got it!.thank you! ...
written 18 months ago by Wang10
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Why ranges of narrowPeak file and that of bed file are not identical
... Hi everyone! I did peak call using MACS2 to analysis a Chip-seq data and I found the ranges in the narrowPeak file is not same as that in the bed file.what dose this mean?and why?Thanks! more ff_peaks.narrowPeak chr1 3044565 3044674 ff_peak_1 97 . 3.97001 12.89295 ...
macs2 chip-seq sequencing written 18 months ago by Wang10 • updated 18 months ago by ATpoint42k
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Comment: C: If we use MACS2 do we need to remove duplicate sequences with samtools rmdup ?
... That's interesting! I removed duplications by samtools rmdup while after peak calling using MACS2 I got the same results as that without remove duplications wc -l ko_*.narrowPeak 125573 ko_nodup_peaks.narrowPeak 125573 ko_peaks.narrowPeak So "since MACS2 will ignore them anyway yo ...
written 18 months ago by Wang10 • updated 18 months ago by GenoMax92k
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Comment: C: Analysis of RNA-seq data of Ion Torrent
... Thank you so much for those useful information! I really appreciate it! ...
written 19 months ago by Wang10
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Comment: C: Analysis of RNA-seq data of Ion Torrent
... I'd like do differential expression analysis.Or further pathway clustering,network analysis if possible. ...
written 19 months ago by Wang10

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Supporter 16 months ago, voted at least 25 times.

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