User: Michael
Michael • 160
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- Swizerland
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- https://www.genexa.ch/
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- Last seen:
- 3 days, 2 hours ago
- Joined:
- 1 year, 8 months ago
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- m*************@genexa.ch
Posts by Michael
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Comment:
C: ABySS vs. MaSuRCA -- opinions
... This is a pretty clear answer ;) Just wondering, did you ever do Illumina only with MaSuRCA? ...
written 6 weeks ago by
Michael • 160
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... What is your opinion on ABySS vs. MaSuRCA for Illumina only assemblies (*de novo*)?
I have:
- Genomes about 1GB in size
- Di- or Tetraploid
- About 60x coverage
- Paired-end 150bp Illumina
Would you go for ABySS or MaSuRCA? Or do you even have another one you would pick?
I think ABySS should ...
written 6 weeks ago by
Michael • 160
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... I need a tool to design crRNAs (Cas9) for no-Amp enrichment. It will be used for PacBio or Oxford Nanopore.
An example for an application is here: https://www.pacb.com/wp-content/uploads/Reference-Guide-Designing-CRISPR-Cas9-RNA-Oligonucleotides-for-the-No-Amp-Targeted-Sequencing-Procedure.pdf
The ...
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... Universities are funded by public money (mostly) -- so should be open. Those places are not there to monopolize data!
Private companies are a different thing. Do not mix those things. Although many companies share data as well because they see that it helps them as well. ...
written 9 months ago by
Michael • 160
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... I guess what would solve the problem is if GISAID would allow INSDC to sync data across their databases. Which is not possible the way GISAID operates at the moment.
Fun thing is: GISAID imports data from open INSDC databases and incorporate it into their EpiFlu database ;) ...
written 9 months ago by
Michael • 160
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... Don't think I am allowed to... ...
written 10 months ago by
Michael • 160
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... If you have access to GISAID you can download the acknowledgement table. There you can identify labs/hospitals uploading data to GISAID. You can contact them and suggest that they (also) upload to GenBank/ENA/etc.
I just did that, let's see what response I get ;) ...
written 10 months ago by
Michael • 160
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... Istvan, 100% agree. I think the way NCBI, ENA and others handle this, provides the same level of acknowledgment.
To me it looks a bit like this is just some way of monopolizing the data in order to still be "important". ...
written 10 months ago by
Michael • 160
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... Istvan, this was my thinking as well! Why is it necessary to execute "dos2unix" first. WTF. :(
One should really encourage every single researcher to submit to NCBI, ENA, et al. ...
written 10 months ago by
Michael • 160
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... OK, now it is there... It was not so far.
Maybe you have to specifically ask for it. I wrote them a mail yesterday that batch download would be very helpful. They might have added a flag for batch download now. Whatever...
EDIT: Thanks for the script by the way!! ...
written 10 months ago by
Michael • 160
Latest awards to Michael
Popular Question
6 months ago,
created a question with more than 1,000 views.
For How to get BLAST wgs database
Popular Question
6 months ago,
created a question with more than 1,000 views.
For A resource with diverse databases and tools for SARS-CoV-2 (COVID-19) bioinformatics -- Updates
Appreciated
10 months ago,
created a post with more than 5 votes.
For A resource with diverse databases and tools for SARS-CoV-2 (COVID-19) bioinformatics
Scholar
20 months ago,
created an answer that has been accepted.
For A: Databases to blast against when checking for human sequences
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