User: juanjo75es

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juanjo75es70
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Posts by juanjo75es

<prev • 34 results • page 1 of 4 • next >
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Comment: C: construction of draft genome from the contigs
... I think your data is not well issued for de novo assembly. There are no overlapping regions between the reads. The only overlapping regions are chimeras generated by the PCR. The one who designed the library used a library preparation that is valid for reference alignment of the reads but not for de ...
written 23 days ago by juanjo75es70
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Comment: C: construction of draft genome from the contigs
... Hi liorgiic, could I know exactly what run you used to obtain that N50? I would like to make some tests (just for learning). I have tried SRR3107830 with Spades, for example, and I only get contigs under 500 bp length. ...
written 23 days ago by juanjo75es70
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Comment: C: Multiple alignment software
... Just some updates given that people seem to be quite interested on that topic. (I) I didn't read all comments. Too much nonsesnse IMHO. I will try to focus only on what is interesting for me. Some people here seem to be very young and/or lacking many vital experiences. That's the better explanation ...
written 12 months ago by juanjo75es70
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Comment: C: Multiple alignment software
... Could someone explain me why my answer (the one I consider the right one) was moved to a comment while a deeply wrong answer is keeped? ...
written 12 months ago by juanjo75es70
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Comment: C: Multiple alignment software
... Again you make a deeply unaccurate statement "if we can explain it adequately with 2-3 events". You need a lot more events to explain their alignment. Indeed you need more events than with mine. Unless you believe that DNA replication makes a lot of transcription mistakes in just one event. It's qui ...
written 12 months ago by juanjo75es70
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Comment: C: Multiple alignment software
... Mensur, thanks for replying again. I guessed you realized from the last time that I didn't agree with your reply. I don't think it's the point of this forum to discuss philosophical or political issues. That's why I didn't explain you last time why I think you are deeply wrong. I won't either do thi ...
written 12 months ago by juanjo75es70
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Comment: C: Multiple alignment software
... I am not aligning genes... Biology is not only about genes. The biomedical industry is what seems to be only about genes. People here are biased, And they don't seem to take seriously any scientifical method. Sorry if I generalize. People who do not agree with all bullshit displayed here should say ...
written 12 months ago by juanjo75es70
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Comment: A: Multiple alignment software
... As I take from some comments, looks like the problem here is that my algorithm and the other ones are designed for different goals. Mine is (actually) conceived to align non-conserved sequences while the other two are mostly designed to align conserved regions at which INDELs are less frequent than ...
written 12 months ago by juanjo75es70
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Comment: C: Multiple alignment software
... > Not sure why you write something like this That usually helps to instantly identify those who do not have honest intentions writing an answer. They usually lose their cool for no apparent reason. Apart from that it's (more or less) the truth and it's usually healthy to display the truth . Glad ...
written 12 months ago by juanjo75es70
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Comment: C: Multiple alignment software
... So, finding two almost dentical SINE sequences in two different places of a genome and assuming they are related means "Sorry, but this is a totally wrong assumption". Could you expand on that? ...
written 12 months ago by juanjo75es70

Latest awards to juanjo75es

Popular Question 10 months ago, created a question with more than 1,000 views. For Multiple alignment software
Scholar 12 months ago, created an answer that has been accepted. For A: Multiple alignment software

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