Moderator: Brad Chapman
Brad Chapman ♦ 9.4k
- Reputation:
- 9,390
- Status:
- Trusted
- Location:
- Boston, MA
- Website:
- http://bcbio.wordpress...
- Twitter:
- chapmanb
- Last seen:
- 5 months, 3 weeks ago
- Joined:
- 9 years, 3 months ago
- Email:
- c*******@50mail.com
Biologist and programmer at Harvard School of Public Health
Posts by Brad Chapman
<prev
• 325 results •
page 1 of 33 •
next >
0
votes
4
answers
720
views
4
answers
... Thanks for this report and apologies for missing this when initially posted. I was able to reproduce this problem and sent in a pull request to CNVkit that should fix the problem: https://github.com/etal/cnvkit/pull/393 Hope this small fix will work for you and comes in a reasonable time to help wit ...
written 8 months ago by
Brad Chapman ♦ 9.4k
0
votes
1
answer
1.3k
views
1
answers
... Thanks for this pointer. As an additional data point, I ran into a similar issue in a restricted build environment for bioconda and adding perl to the environment fixes it for me. ...
written 2.4 years ago by
Brad Chapman ♦ 9.4k
1
vote
1
answer
1.3k
views
1
answers
... For processing with sra you'll want to convert into standard fastq format using the SRA toolkit (http://www.ncbi.nlm.nih.gov/books/NBK158900/). In bcbio, you can add a custom reference genome for tuberculosis (https://bcbio-nextgen.readthedocs.org/en/latest/contents/configuration.html#adding-custom- ...
written 3.5 years ago by
Brad Chapman ♦ 9.4k
2
votes
1
answer
1.3k
views
1
answers
... It's possible to include it, but it would need integration work to include it as a new aligner. Documentation on writing that code is here:
https://bcbio-nextgen.readthedocs.org/en/latest/contents/code.html#aligner
Out of curiousity, why do you prefer BBMap over other integrated aligners in bcbio ...
written 3.5 years ago by
Brad Chapman ♦ 9.4k
0
votes
2
answers
1.5k
views
2
answers
... Steve is right that the Genome in a Bottle group is still working on a native 38 build of the reference. In the short term, we've been doing work on providing a Remapped/Crossmapped version that converts the 37 reference to 38 coordinates. This isn't finished as I'm not happy with the Remapping of S ...
written 3.9 years ago by
Brad Chapman ♦ 9.4k
5
votes
3
answers
4.4k
views
3
answers
Answer:
A: Tumor clonality algorithms
... We've been working on integrating clonality and heterogeneity estimation tools into bcbio (https://github.com/chapmanb/bcbio-nextgen). It's still a work in progress but we've been evaluating against internal datasets where we have external predictions of normal contamination. We've had the most succ ...
written 4.0 years ago by
Brad Chapman ♦ 9.4k
9
votes
3
answers
3.3k
views
3
answers
... Shane;
Welcome to bioinformatics work. It would be easier to offer suggestions with an idea of your programming background and biological interests. As a starting point, the ADAM project has a nice contributing guide, great group of folks, and the issue tracker has 'Pick Me Up!' issues which are me ...
written 4.4 years ago by
Brad Chapman ♦ 9.4k
3
votes
1
answer
4.6k
views
1
answers
... Pierre;
The source code is here:
https://github.com/konradjk/exac_browser
It looks like a MongoDB database and Python Flask webserver. It also builds on xBrowse:
https://github.com/xbrowse/xbrowse
...
written 4.4 years ago by
Brad Chapman ♦ 9.4k
3
votes
4
answers
3.5k
views
4
answers
... The approach isn't species specific. The general idea is to find regions where we can reliably break analysis steps into chunks that are smaller than whole chromosomes and as balanced as possible. We do this in bcbio and it has been used across a wide variety of species without issues.
Another tool ...
written 4.4 years ago by
Brad Chapman ♦ 9.4k
7
votes
4
answers
3.5k
views
4
answers
... This is absolutely possible, and as Devon mentioned similar to approaches we use in bcbio (https://github.com/chapmanb/bcbio-nextgen). In bcbio we haven't yet worked on single sample optimization. Practically we don't have users with a large number of idle cores and the use case of pushing through a ...
written 4.4 years ago by
Brad Chapman ♦ 9.4k
Latest awards to Brad Chapman
Cylon
2.2 years ago,
received 1,000 up votes.
Good Answer
2.2 years ago,
created an answer that was upvoted at least 5 times.
For A: Ngs Challenge: Can You Give An Insight About This Imaginary Genetic Disease ?
Appreciated
2.2 years ago,
created a post with more than 5 votes.
For A: Gatk Hardyweinberg Calculation
Appreciated
2.7 years ago,
created a post with more than 5 votes.
For A: Gatk Hardyweinberg Calculation
Appreciated
2.9 years ago,
created a post with more than 5 votes.
For A: Gatk Hardyweinberg Calculation
Scholar
3.4 years ago,
created an answer that has been accepted.
For A: Exome Aggregation Consortium (ExAC) database ?
Teacher
3.4 years ago,
created an answer with at least 3 up-votes.
For A: Should I Separate The Snp And Indel When Performing The Gatk Vqsr?
Appreciated
3.4 years ago,
created a post with more than 5 votes.
For A: Gatk Hardyweinberg Calculation
Appreciated
3.5 years ago,
created a post with more than 5 votes.
For A: Gatk Hardyweinberg Calculation
Scholar
3.8 years ago,
created an answer that has been accepted.
For A: Unbelievably rapid NGS analysis by Churchill
Scholar
4.0 years ago,
created an answer that has been accepted.
For A: Unbelievably rapid NGS analysis by Churchill
Teacher
4.0 years ago,
created an answer with at least 3 up-votes.
For A: Should I Separate The Snp And Indel When Performing The Gatk Vqsr?
Good Answer
4.3 years ago,
created an answer that was upvoted at least 5 times.
For A: Hypergeometric Test R/Bioconductor Result Categorization
Appreciated
4.4 years ago,
created a post with more than 5 votes.
For A: Gatk Hardyweinberg Calculation
Good Answer
4.4 years ago,
created an answer that was upvoted at least 5 times.
For A: Hypergeometric Test R/Bioconductor Result Categorization
Teacher
4.4 years ago,
created an answer with at least 3 up-votes.
For A: Should I Separate The Snp And Indel When Performing The Gatk Vqsr?
Appreciated
4.4 years ago,
created a post with more than 5 votes.
For A: Gatk Hardyweinberg Calculation
Scholar
4.4 years ago,
created an answer that has been accepted.
For A: Unbelievably rapid NGS analysis by Churchill
Teacher
4.4 years ago,
created an answer with at least 3 up-votes.
For A: Should I Separate The Snp And Indel When Performing The Gatk Vqsr?
Good Answer
4.4 years ago,
created an answer that was upvoted at least 5 times.
For A: Is There A Fast Hashing Function For Nucleotide K-Mers (Q-Grams)?
Appreciated
4.4 years ago,
created a post with more than 5 votes.
For A: Gatk Hardyweinberg Calculation
Use of this site constitutes acceptance of our User
Agreement
and Privacy
Policy.
Powered by Biostar
version 2.3.0
Traffic: 1837 users visited in the last hour