User: xiaoguang

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xiaoguang10
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4 days, 22 hours ago
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Posts by xiaoguang

<prev • 6 results • page 1 of 1 • next >
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How to get an exact match from the bam alignment file
... How to get an exact match from the bam comparison file, it is well known that M in the cigar character is completely consistent or there is mismatch, but how do I get a completely consistent sequence? ...
bam samtools bwa written 4 weeks ago by xiaoguang10
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Comment: C: how to caculate effective length for RNA sequencing?
... If we use featurecount, we can get gens quantification beacuse of aligning sequences to genome, but we use Salmon, we only get isoforms quantification. however,TPM is the percentage of FPKM,Are they different? ...
written 5 weeks ago by xiaoguang10
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how to caculate effective length for RNA sequencing?
... Is there any R packages or programs could help me to calculate **effective length** of every isoform or gene? We know the importance of effective length when we calculate TPM for isoforms or genes . but we can not got it from featurecount program. Salmon can get it ,but it is only for ...
R rna-seq written 5 weeks ago by xiaoguang10 • updated 6 days ago by Gordon Smyth950
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Comment: C: proj_api.h not found in standard or given locations
... Thank you very much, I know what's wrong with me. I have not installed proj.4. ...
written 5 weeks ago by xiaoguang10
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proj_api.h not found in standard or given locations
... When I install R package "sf", I always meet this error: > configure: error: proj_api.h not found in standard or given locations." Actually, I have "proj_api.h" in my miniconda3 includ dir and "proj_api.so"in lib dir, but it continue give me an error? ...
R miniconda written 5 weeks ago by xiaoguang10 • updated 5 weeks ago by zx87548.0k
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How to normalized TPM with TMM method?
... We can use edgeR to normalized raw read counts with TMM method, but how can we normalized TPM with the same method? ...
tpm rna-seq edger tmm written 6 weeks ago by xiaoguang10 • updated 8 days ago by Gordon Smyth950

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