User: Samuel

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Samuel10
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Posts by Samuel

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Comment: C: Making genome-wide transcription level track from transcript level count
... I see, thank you very much for the explanation, it is very helpful! ...
written 16 hours ago by Samuel10
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Making genome-wide transcription level track from transcript level count
... I used Salmon to quantify RNA at the transcript level. Is there a way to map this data into a format that can be viewed in IGV? A related question, i also tried another method of quantifying where i aligned the read with STAR and counted with GenomicAlignments. The counts from this method is easi ...
assembly next-gen rna-seq written 5 days ago by Samuel10 • updated 5 days ago by caggtaagtat780
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bigWigAverageOverBed not accepting bed file
... I'm trying to construct an IGV track that lets me visualise genome wide RNA expression level but i'm running into some problems trying to bin the data. This is what i did: - made a .bed file using `bedtools makewindow` with chrom.sizes as input - generated a bedgraph with `bedtools genomecov` - co ...
software error alignment rna-seq sequencing written 10 days ago by Samuel10
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Mapping C albicans supercontigs to chromosomes
... Hello, Apologies if this is a silly questions, I am still new to bioinformatics and learning as I go. I am trying to generate a BDG file to visualise RNA transcription levels of WT SC5314 C.albicans in IGV. I am following [this][1] manual. I understand that i will need a GTF/GFF file to serve as a ...
rna-seq c_albicans written 13 days ago by Samuel10

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