User: dextereyes88

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dextereyes8810
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Posts by dextereyes88

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Answer: A: How I deal with raw read counts of RNA-seq
... I prefer to use the bitr function in the clusterProfiler package for mapping gene symbols to ensembl IDs. It will tell you if there is 1:1 matching or many:1 matching, and will print the percentage of non-mapped IDs. [https://yulab-smu.github.io/clusterProfiler-book/chapter14.html#bitr][1] It is c ...
written 11 days ago by dextereyes8810
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pbalign and SMRT reads
... Hello, I recently used exonerate to align PacBio Iso-Seq SMRT reads. Although this worked, it was very slow, and the data sets I had were pretty small (only about 3400 reads). I now have new, much larger data sets (~70,000 reads) and exonerate is just not cutting it. I have to split the fasta files ...
genome alignment rna-seq sequencing written 11 days ago by dextereyes8810

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