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Posts by Jana
Comment: C: Read alignment using Bowtie2
... Hi, Thank you [ATpoint] for your suggestion. So this is related to CRISPR-CAS9. I am working with off-target predictions for my thesis and was looking at all scientific papers related to CRISPR. I found one and decided to use their datasets. The datasets were not available directly and the me ...
... Hello everyone! I am in need of using Bowtie2 to find all possible alignments for a set of reads (each 23bp long) to the human genome. The maximum number of mismatches that can be tolerated is 6. But since Bowtie2 allows mismatches only upto 1, I had to set the mismatch penalty for alignment score ...
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