User: segrossk

gravatar for segrossk
segrossk0
Reputation:
0
Status:
New User
Last seen:
1 month ago
Joined:
1 month ago
Email:
s*******@hotmail.com

Profile information, website and location are not shown for new users.

This helps us discourage the inappropriate use of our site.

Posts by segrossk

<prev • 3 results • page 1 of 1 • next >
0
votes
1
answer
222
views
1
answers
Comment: C: bcl2fastq with xGen Dual Index UMI Adapters to produce 3 read and 2 index fastqs
... genomax, adding the --mask-short-adapter-reads 0 option to the bcl2fastq commands in the original post worked perfectly. I would not have thought of using that option. Thank you so much! ...
written 4 weeks ago by segrossk0
0
votes
1
answer
222
views
1
answers
Comment: C: bcl2fastq with xGen Dual Index UMI Adapters to produce 3 read and 2 index fastqs
... genomax: Thank you for pointing out your post, which I came across and it did not resolve my issue of bcl2fastq placing 9bp N's instead of the sequence in the UMI files. The options that I use for bcl2fastq does everything correct except for the R2 fastq containing the UMI read. The R1 and R4 76bp r ...
written 4 weeks ago by segrossk0
3
votes
1
answer
222
views
5 follow
1
answer
bcl2fastq with xGen Dual Index UMI Adapters to produce 3 read and 2 index fastqs
... I have RNA-Seq from Illumina HiSeq using IDT xGen Dual Index UMI Adapters, where the UMI are added on the i7 index. Typically, I would use a pipeline using Picard which is recommended by IDT. However, I was hoping to use bcl2fastq (v2.17.1.14) to immediately produce fastqs. Below is the molecular b ...
rna-seq written 4 weeks ago by segrossk0 • updated 4 weeks ago by swbarnes26.7k

Latest awards to segrossk

No awards yet. Soon to come :-)

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 2574 users visited in the last hour